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Literature summary for 1.13.99.1 extracted from
- Biochemical studies on inositol. V. Purification and properties of the enzyme that cleaves inositol to D-glucuronic acid (1959), J. Biol. Chem., 234, 220-227.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | best activation system: 1 mM Fe(II) and 2 mM cysteine | Rattus norvegicus |  |
| quinolinate | 1 mM Fe(II) + 4 mM quinolinate activates to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Thenoyltrifluoroacetone | slight | Rattus norvegicus | |
| 8-hydroxyquinoline | - |
Rattus norvegicus | |
| ADP | slight | Rattus norvegicus | |
| Ag+ | - |
Rattus norvegicus | |
| AMP | - |
Rattus norvegicus | |
| arsenite | - |
Rattus norvegicus | |
| ATP | - |
Rattus norvegicus | |
| azide | - |
Rattus norvegicus | |
| CTP | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| cyanide | - |
Rattus norvegicus | |
| FAD | - |
Rattus norvegicus | |
| Furoylthiofluoroacetone | slight | Rattus norvegicus | |
| GTP | - |
Rattus norvegicus | |
| Hg2+ | - |
Rattus norvegicus | |
| hydroxylamine | - |
Rattus norvegicus | |
| iodoacetate | - |
Rattus norvegicus | |
| menadione | - |
Rattus norvegicus | |
| NAD+ | - |
Rattus norvegicus | |
| NADP+ | - |
Rattus norvegicus | |
| o-phenanthroline | slight | Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
| Phenylmercuric nitrate | - |
Rattus norvegicus | |
| Quinacrine hydrochloride | slight | Rattus norvegicus | |
| riboflavin phosphate | - |
Rattus norvegicus | |
| tetrahydrofolic acid | - |
Rattus norvegicus | |
| Uridine diphosphoglucose | - |
Rattus norvegicus | |
| UTP | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.1 | - |
Inositol | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | best activation system | Rattus norvegicus | |
| Fe2+ | 1 mM Fe(II) + 4 mM quinolinate activate to 70% of the Fe(II)/cysteine system, Fe(II) alone causes very little activation, quinolinate gives considerable activation in absence of Fe(II), activation by Fe(II) and quinolinate is very temperature dependent | Rattus norvegicus | |
| Fe2+ | 1 mM Fe(II) and 2 mM cysteine | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, extensive loss of activity after 1 or 2 days | Rattus norvegicus |
| 0°C, 12 h, extensive loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | highly specific for myo-inositol | Rattus norvegicus | D-glucuronate + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.22 | - |
Inositol | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.2 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.4 | sharp decrease of activity below pH 6.5 and above pH 7.4 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | 5. 6 mMol per mol of enzyme | Rattus norvegicus | |
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