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Literature summary for 1.13.12.7 extracted from

  • Moradi, A.; Hosseinkhani, S.; Naderi-Manesh, H.; Sadeghizadeh, M.; Alipour, B.S.
    Effect of charge distribution in a flexible loop on the bioluminescence color of firefly luciferases (2009), Biochemistry, 48, 575-582.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Photinus pyralis

Protein Variants

Protein Variants Comment Organism
ins356E green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 97% increase in specific activity Photinus pyralis
ins356K green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 18% decrease in specific activity Photinus pyralis
ins356Q at pH 5.5 red-emitting luciferase, increase in thermostability, 6% decrease in specific activity Photinus pyralis
ins356R green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 12% decrease in specific activtiy Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
D-luciferin mutant ins356E Photinus pyralis
0.009
-
D-luciferin mutant ins356K Photinus pyralis
0.011
-
D-luciferin wild-type luciferase Photinus pyralis
0.015
-
D-luciferin mutant ins356R Photinus pyralis
0.018
-
D-luciferin mutant ins356Q Photinus pyralis
0.1
-
ATP mutant ins356R Photinus pyralis
0.105
-
ATP mutant ins356E Photinus pyralis
0.105
-
ATP mutant ins356Q Photinus pyralis
0.11
-
ATP mutant ins356K Photinus pyralis
0.12
-
ATP wild-type luciferase Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
SDS-PAGE Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis P08659 naturally green-emitting luciferase at pH 7.8
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose, yield of 95% Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + ATP + O2
-
Photinus pyralis luciferyl-adenylate + diphosphate
-
?
luciferyl-adenylate
-
Photinus pyralis oxyluciferin + hnu
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Photinus pyralis
Ppy
-
Photinus pyralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
complete inactivation, mutant ins356Q still 20% residual activity Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
shift of emission spectra to red region Photinus pyralis
7.8
-
-
Photinus pyralis