Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
ins356E | green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 97% increase in specific activity | Photinus pyralis |
ins356K | green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 18% decrease in specific activity | Photinus pyralis |
ins356Q | at pH 5.5 red-emitting luciferase, increase in thermostability, 6% decrease in specific activity | Photinus pyralis |
ins356R | green and red emitting light at pH 7.8, at pH 5.5 red-emitting luciferase, 12% decrease in specific activtiy | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-luciferin | mutant ins356E | Photinus pyralis | |
0.009 | - |
D-luciferin | mutant ins356K | Photinus pyralis | |
0.011 | - |
D-luciferin | wild-type luciferase | Photinus pyralis | |
0.015 | - |
D-luciferin | mutant ins356R | Photinus pyralis | |
0.018 | - |
D-luciferin | mutant ins356Q | Photinus pyralis | |
0.1 | - |
ATP | mutant ins356R | Photinus pyralis | |
0.105 | - |
ATP | mutant ins356E | Photinus pyralis | |
0.105 | - |
ATP | mutant ins356Q | Photinus pyralis | |
0.11 | - |
ATP | mutant ins356K | Photinus pyralis | |
0.12 | - |
ATP | wild-type luciferase | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | P08659 | naturally green-emitting luciferase at pH 7.8 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose, yield of 95% | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + ATP + O2 | - |
Photinus pyralis | luciferyl-adenylate + diphosphate | - |
? | |
luciferyl-adenylate | - |
Photinus pyralis | oxyluciferin + hnu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
Ppy | - |
Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
complete inactivation, mutant ins356Q still 20% residual activity | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
shift of emission spectra to red region | Photinus pyralis |
7.8 | - |
- |
Photinus pyralis |