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Literature summary for 1.13.12.7 extracted from

  • Baggett, B.; Roy, R.; Momen, S.; Morgan, S.; Tisi, L.; Morse, D.; Gillies, R.J.
    Thermostability of firefly luciferases affects efficiency of detection by in vivo bioluminescence (2004), Mol. Imaging, 3, 324-332.
    View publication on PubMed

Application

Application Comment Organism
medicine luciferase gene is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression. The use of thermostabilized luciferases may allow monitoring of micro-metastses and the early stages of tumor growth Photinus pyralis

Cloned(Commentary)

Cloned (Comment) Organism
-
Photinus pyralis

Protein Variants

Protein Variants Comment Organism
E345K/A215L half-life at 37°C is 7.36 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis
E345K/A215L/I232A/T214A half-life at 37°C is 75.1 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis
E345K/A215L/I232A/T214A/F295L half-life at 37°C is 82.1 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis
E345K/I232A/T214A half-life at 37°C is 15.5 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis
E345K/I232A/T214A/F295L/S420T half-life at 37°C is 72.4 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis
E345K/T214A half-life at 37°C is 8.5 min, compared to 3.06 min for the wild-type enzyme Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
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Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life: 3.06 min for wild-type enzyme,8.5 min for mutant enzyme E345K/T214A, 15.5 min for E345K/I232A/T214A, 7.36 min for E345K/A215L, 72.4 min for E345K/I232A/T214A/F295L/S420T, 82.1 min for E345K/A215L/I232A/T214A/F295L, 75.1 min for E345K/A215L/I232A/T214A Photinus pyralis