Literature summary for 1.13.12.7 extracted from

Branchini, B.R.; Southworth, T.L.; Murtiashaw, M.H.; Wilkinson, S.R.; Khattak, N.F.; Rosenberg, J.C.; Zimmer, M.
Mutagenesis evidence that the partial reactions of firefly bioluminescence are catalyzed by different conformations of the luciferase C-terminal domain (2005), Biochemistry, 44, 1385-1393.

Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant enzymes expressed as glutathione-S-transferase-fusion proteins containing the additional N-terminal peptide Gly-Pro-Leu-Gly-SER, which remains after PreScission protease cleavage from GST, expression in Escherichia coli	Photinus pyralis

Protein Variants

Protein Variants	Comment	Organism
G446I	turnover number is 8.4fold lower compared to wild-type value, KM-value for D-luciferin is 1.5fold lower compared to wild-type value, KM-value for MgATP2- is 2.2fold lower compared to wild-type value, the bioluminescence emission maximum is 554 nm, compared to 558 nm for the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 5.1fold lower compared to wild-type value	Photinus pyralis
K443A	turnover number is 2655 fold lower compared to wild-type value, KM-value for D-luciferin is 6.5fold lower compared to wild-type value, KM-value for MgATP2- is 3.3fold lower compared to wild-type value, the bioluminescence emission maximum is identical to the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 181fold lower compared to wild-type value	Photinus pyralis
K443A/K529A	turnover number is 1063700fold lower compared to wild-type value, KM-value for D-luciferin is 4.5fold higher compared to wild-type value, KM-value for MgATP2- is 3.5fold higher compared to wild-type value, the bioluminescence emission maximum is 596 nm, compared to 558 nm for the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 858fold lower compared to wild-type value	Photinus pyralis
K445Q	turnover number is 1.4fold higher compared to wild-type value, KM-value for D-luciferin is 1.7fold lower compared to wild-type value, KM-value for MgATP2- is 2.3fold lower compared to wild-type value, the bioluminescence emission maximum is 556 nm, compared to 558 nm for the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 1.7fold higher compared to wild-type value	Photinus pyralis
K529A	turnover number is 668fold lower compared to wild-type value, KM-value for D-luciferin is 15.3fold higher compared to wild-type value, KM-value for MgATP2- is 7.5fold higher compared to wild-type value, the bioluminescence emission maximum is 562 nm, compared to 558 nm for the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 3.5fold lower compared to wild-type value	Photinus pyralis
Q448A	turnover number is 1.9fold lower compared to wild-type value, KM-value for D-luciferin is 2.5fold lower compared to wild-type value, KM-value for MgATP2- is 2.1fold higher compared to wild-type value, the bioluminescence emission maximum is 557 nm, compared to 558 nm for the wild-type value. The ratio of turnover-number to KM-value for D-luciferyl-O-adenosine monophosphate is 1.1fold lower compared to wild-type value	Photinus pyralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.00038	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K443A	Photinus pyralis
0.00055	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K443A/K529A	Photinus pyralis
0.0015	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme G446I	Photinus pyralis
0.0022	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme Q448A	Photinus pyralis
0.0023	-	D-luciferin	mutant enzyme K443A	Photinus pyralis
0.0039	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K445Q	Photinus pyralis
0.0047	-	D-luciferyl-O-adenosine monophosphate	wild-type enzyme	Photinus pyralis
0.006	-	D-luciferin	mutant enzyme G446I	Photinus pyralis
0.006	-	D-luciferin	mutant enzyme Q448A	Photinus pyralis
0.0089	-	D-luciferin	mutant enzyme K445Q	Photinus pyralis
0.0126	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K529A	Photinus pyralis
0.015	-	D-luciferin	wild-type enzyme	Photinus pyralis
0.0486	-	MgATP2-	mutant enzyme K443A	Photinus pyralis
0.067	-	D-luciferin	mutant enzyme K443A/K529A	Photinus pyralis
0.069	-	MgATP2-	mutant enzyme K445Q	Photinus pyralis
0.073	-	MgATP2-	mutant enzyme G446I	Photinus pyralis
0.16	-	MgATP2-	wild-type enzyme	Photinus pyralis
0.23	-	D-luciferin	mutant enzyme K529A	Photinus pyralis
0.341	-	MgATP2-	mutant enzyme Q448A	Photinus pyralis
0.56	-	MgATP2-	mutant enzyme K443A/K529A	Photinus pyralis
1.2	-	MgATP2-	mutant enzyme K529A	Photinus pyralis

Organism

Organism	UniProt	Comment	Textmining
Photinus pyralis	P08659	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-luciferin + O2 + ATP	-	Photinus pyralis	oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv	-	?
D-luciferyl-O-adenosine monophosphate + ?	-	Photinus pyralis	?	-	?
luciferin + MgATP2-	-	Photinus pyralis	AMP + diphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.000000157	-	MgATP2-	mutant enzyme K443A/K529A	Photinus pyralis
0.000000157	-	D-luciferin	mutant enzyme K443A/K529A	Photinus pyralis
0.0000311	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K443A/K529A	Photinus pyralis
0.0000629	-	MgATP2-	mutant enzyme K443A	Photinus pyralis
0.0000629	-	D-luciferin	mutant enzyme K443A	Photinus pyralis
0.000101	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K443A	Photinus pyralis
0.00025	-	MgATP2-	mutant enzyme K529A	Photinus pyralis
0.00025	-	D-luciferin	mutant enzyme K529A	Photinus pyralis
0.02	-	MgATP2-	mutant enzyme G446I	Photinus pyralis
0.02	-	D-luciferin	mutant enzyme G446I	Photinus pyralis
0.021	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme G446I	Photinus pyralis
0.0875	-	MgATP2-	mutant enzyme Q448A	Photinus pyralis
0.0875	-	D-luciferin	mutant enzyme Q448A	Photinus pyralis
0.095	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme Q448A	Photinus pyralis
0.167	-	MgATP2-	wild-type enzyme	Photinus pyralis
0.167	-	D-luciferin	wild-type enzyme	Photinus pyralis
0.175	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K529A	Photinus pyralis
0.23	-	D-luciferyl-O-adenosine monophosphate	wild-type enzyme	Photinus pyralis
0.24	-	MgATP2-	mutant enzyme K445Q	Photinus pyralis
0.24	-	D-luciferin	mutant enzyme K445Q	Photinus pyralis
0.32	-	D-luciferyl-O-adenosine monophosphate	mutant enzyme K445Q	Photinus pyralis