Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | activator of yellow-green light at pH 6.5 | Photinus pyralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole | competitive inhibitor | Photinus pyralis | |
5-anilino-1-naphthalene sulfonate | - |
Photinus pyralis | |
6-toluidino-2-naphthalene sulfonate | - |
Photinus pyralis | |
ATP | uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex | Photinus pyralis | |
dehydroluciferin | - |
Photinus pyralis | |
N-ethylmaleimide | - |
Photinus pyralis | |
N-tosyl-L-phenylalanine chloromethyl ketone | - |
Photinus pyralis | |
p-mercuribenzoate | - |
Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | increasing concentration shifts the light wavelenghts to higher values | Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
- |
Photinus pyralis |
92000 | 95000 | high speed equilibrium sedimentation | Photinus pyralis |
100000 | - |
from sedimentation velocity of the crystalline form | Photinus pyralis |
500000 | - |
2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme | Photinus pyralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Photinus luciferin + O2 + ATP | Photinus pyralis | highly specific for ATP | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
quantum yield 0.88 with respect to luciferin | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dimethylluciferin + ATP + O2 | produces only red light, 6-amino and 4 hydroxy analogs tested | Photinus pyralis | dehydroluciferin-adenylate + diphosphate | - |
? | |
dehydroluciferin + ATP + O2 | - |
Photinus pyralis | diphosphate + dehydroluciferin-adenylate | - |
? | |
luciferin + ATP + O2 | - |
Photinus pyralis | oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
r | |
luciferin + MgATP2- | - |
Photinus pyralis | AMP + diphosphate | - |
? | |
Photinus luciferin + O2 + ATP | highly specific for ATP | Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
Photinus luciferin + O2 + ATP | the true substrate is MgATP2- | Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | two non identical subunits | Photinus pyralis |
dimer | 2 * 500000 sedimentation equilibrium study of guanidine hydrochloride-treated enzyme | Photinus pyralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | pH up to 8 red light occurs instead of yellow-green light | Photinus pyralis |
7.5 | 8 | pH below 7.5 emission shifts to longer wavelengths | Photinus pyralis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
dehydroluciferin | - |
Photinus pyralis | |
0.0006 | - |
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole | - |
Photinus pyralis |