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Literature summary for 1.13.12.6 extracted from

  • Nakajima, Y.; Kobayashi, K.; Yamagishi, K.; Enomoto, T.; Ohmiya, Y.
    cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca (2004), Biosci. Biotechnol. Biochem., 68, 565-570.
    View publication on PubMed
Search for more literature for 1.13.12.6

Application

Application Comment Organism
biotechnology use of enzyme as a potent secreted reporter Cypridina noctiluca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine pH 7.4 Cypridina noctiluca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61415
-
 x * 61415, deduced from gene sequence Cypridina noctiluca

Organism

Organism UniProt Comment Textmining
Cypridina noctiluca Q75R40
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Cypridina noctiluca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine + O2
-
 Cypridina noctiluca N-[3-(3-carbamimidamidopropyl)-5-(1H-indol-3-yl)-1,4-dihydropyrazin-2-yl]-2-methylbutanamide + CO2 + hn
-
 ?

Subunits

Subunits Comment Organism
? x * 61415, deduced from gene sequence Cypridina noctiluca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 53 h, 50% residual activity Cypridina noctiluca

pI Value

Organism Comment pI Value Maximum pI Value
Cypridina noctiluca calculated
-
 4.9