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Literature summary for 1.13.12.5 extracted from
- Studies of dynamic protein-protein interactions in bacteria using renilla luciferase complementation are undermined by nonspecific enzyme inhibition (2012), PLoS ONE, 7, e43175.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | split luciferase complementation is applied to study dynamic protein-protein interactions in live bacteria. Nonspecific inhibition of Rluc activity by small molecule effectors compromises the utility of this technique in measuring dynamic protein-protein interactions | Renilla reniformis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fragments of Renilla luciferase (Rluc) are fused to the chemotaxis-associated response regulator CheY3 and its phosphatase CheZ in the enteric pathogen Vibrio cholerae in order to demonstrate dynamic protein-protein interactions by luciferase complementation | Renilla reniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Renilla reniformis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coelenterazine + O2 | - |
Renilla reniformis | coelenteramide + CO2 + hv | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| luciferase | - |
Renilla reniformis |
| RLuc | - |
Renilla reniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Renilla reniformis |
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Release 2023.1 (January 2023)
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