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Literature summary for 1.13.12.5 extracted from

  • Woo, J.; v. Arnim, A.G.
    Mutational optimization of the coelenterazine-dependent luciferase from Renilla (2008), Plant Methods, 4, 23.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology popular reporter enzyme for gene expression and biosensor applications Renilla reniformis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Arabidopsis Renilla reniformis
expression in Escherichia coli strain BL21 Renilla reniformis

Protein Variants

Protein Variants Comment Organism
K189V increased activity Renilla reniformis
M185G slightly increased half life Renilla reniformis
M185V increased activity Renilla reniformis
P220G only 4% of the initial luciferase activity of wild-type luciferase Renilla reniformis
P220L only 16% of the initial luciferase activity of wild-type luciferase Renilla reniformis
V267I increased activity Renilla reniformis

Inhibitors

Inhibitors Comment Organism Structure
coelenteramide
-
Renilla reniformis
additional information loss of enzymatic activity when in contact with substrate Renilla reniformis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Renilla reniformis 5829
-

Organism

Organism UniProt Comment Textmining
Renilla reniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Renilla reniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2 assay at pH 7.2 Renilla reniformis ?
-
?

Synonyms

Synonyms Comment Organism
Renilla luciferase
-
Renilla reniformis
RLuc
-
Renilla reniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
coelenterazine value 111 micromol/min/micromol enzyme Renilla reniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Renilla reniformis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000022
-
coelenteramide
-
Renilla reniformis