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Literature summary for 1.13.12.5 extracted from

  • Inouye, S.
    Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine (2004), FEBS Lett., 577, 105-110.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aequorea forskalea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0133
-
coelenterazine reaction with BFP-aq Aequorea forskalea

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the recombinant protein is expressed in the periplasmic space of Escherichia coli Aequorea forskalea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ after treatment with excess EDTA to remove Ca2+ from BFP-aq, the blue fluorescence shifts to a greenish fluorescence. This greenish fluorescent protein (GFP-aq) is identified as a non-covalent complex of apoaequorin with coelenteramide (oxyluciferin) in a molar ratio of 1:1 Aequorea forskalea

Organism

Organism UniProt Comment Textmining
Aequorea forskalea
-
-
-

Storage Stability

Storage Stability Organism
4°C or -80°C, BFP-aq and gFP-aq do not show significant loss of their fluorescence and luciferase activities after 1 year Aequorea forskalea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2
-
Aequorea forskalea ?
-
?

Synonyms

Synonyms Comment Organism
BFP-aq a complex of Ca2+-bound apoaequorin with coelenteramide Aequorea forskalea
GFP-aq a non-covalent complex of apoaequorin with coelenteramide (oxyluciferin) in a molar ratio of 1:1 Aequorea forskalea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
3 min in the presence of DTT and then cooling on ice, over 93% of the luminescence activity of BFP-aq with coelenterazine is recovered Aequorea forskalea