Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aequorea forskalea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0133 | - |
coelenterazine | reaction with BFP-aq | Aequorea forskalea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the recombinant protein is expressed in the periplasmic space of Escherichia coli | Aequorea forskalea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | after treatment with excess EDTA to remove Ca2+ from BFP-aq, the blue fluorescence shifts to a greenish fluorescence. This greenish fluorescent protein (GFP-aq) is identified as a non-covalent complex of apoaequorin with coelenteramide (oxyluciferin) in a molar ratio of 1:1 | Aequorea forskalea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aequorea forskalea | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C or -80°C, BFP-aq and gFP-aq do not show significant loss of their fluorescence and luciferase activities after 1 year | Aequorea forskalea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coelenterazine + O2 | - |
Aequorea forskalea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFP-aq | a complex of Ca2+-bound apoaequorin with coelenteramide | Aequorea forskalea |
GFP-aq | a non-covalent complex of apoaequorin with coelenteramide (oxyluciferin) in a molar ratio of 1:1 | Aequorea forskalea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
3 min in the presence of DTT and then cooling on ice, over 93% of the luminescence activity of BFP-aq with coelenterazine is recovered | Aequorea forskalea |