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Literature summary for 1.13.12.18 extracted from

  • Schultz, L.W.; Liu, L.; Cegielski, M.; Hastings, J.W.
    Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole (2005), Proc. Natl. Acad. Sci. USA, 102, 1378-1383.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
histidine-tagged Lingulodinium polyedrum luciferase domain 3 is expressed in Escherichia coli BL21(DE3) cells Lingulodinium polyedra

Crystallization (Commentary)

Crystallization (Comment) Organism
Lingulodinium polyedrum luciferase domain 3, vapor diffusion method, using 18-20% (w/v) polyethylene glycol 2000 methyl ether and 100 mM (2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (pH 8.0) Lingulodinium polyedra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lingulodinium polyedra each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light ?
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 ?

Organism

Organism UniProt Comment Textmining
Lingulodinium polyedra O77206
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Purification (Commentary)

Purification (Comment) Organism
nickel metal chelate column chromatography Lingulodinium polyedra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dinoflagellate luciferin + O2
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 Lingulodinium polyedra oxidized dinoflagellate luciferin + H2O + hv
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 ?
additional information each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light Lingulodinium polyedra ?
-
 ?