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Literature summary for 1.13.12.18 extracted from
- Dinoflagellate luciferases: purification of luciferases from Gonyaulax polyedra, Pyrocystis lunula, and Pyrocystis fusiformis (1979), Arch. Biochem. Biophys., 196, 371-384.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Lingulodinium polyedra |  |
| Ag+ | - |
Pyrocystis fusiformis | |
| Ag+ | - |
Pyrocystis lunula | |
| chlorotetracycline | - |
Lingulodinium polyedra | |
| chlorotetracycline | - |
Pyrocystis fusiformis | |
| chlorotetracycline | - |
Pyrocystis lunula | |
| Co2+ | - |
Lingulodinium polyedra | |
| Co2+ | - |
Pyrocystis fusiformis | |
| Co2+ | - |
Pyrocystis lunula | |
| Cu2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| Cu2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Cu2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| cyanide | - |
Lingulodinium polyedra | |
| cyanide | - |
Pyrocystis fusiformis | |
| cyanide | - |
Pyrocystis lunula | |
| EDTA | - |
Lingulodinium polyedra | |
| EDTA | - |
Pyrocystis fusiformis | |
| EDTA | - |
Pyrocystis lunula | |
| EGTA | - |
Lingulodinium polyedra | |
| EGTA | - |
Pyrocystis fusiformis | |
| EGTA | - |
Pyrocystis lunula | |
| Fe2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| Fe2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Fe2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| Fe3+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| Fe3+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Fe3+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| Hg2+ | - |
Lingulodinium polyedra | |
| Hg2+ | - |
Pyrocystis fusiformis | |
| Hg2+ | - |
Pyrocystis lunula | |
| additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Lingulodinium polyedra | |
| additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Pyrocystis fusiformis | |
| additional information | after a 15 min incubation at pH 8.0 and 20°C with subtilisin, trypsin, chymotrypsin, pepsin, carboxypeptidase, and leucine aminopeptidase, the flash height measured in the presence of saturating luciferin is 50% lowered and, after 1 h, the flash height is 10% of the control. Moreover, proteolysis of the extracts completely inactivates the luciferase | Pyrocystis lunula | |
| Ni2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| Ni2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Ni2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
| Pb2+ | - |
Lingulodinium polyedra | |
| Pb2+ | - |
Pyrocystis fusiformis | |
| Pb2+ | - |
Pyrocystis lunula | |
| Sodium azide | - |
Lingulodinium polyedra | |
| Sodium azide | - |
Pyrocystis fusiformis | |
| Sodium azide | - |
Pyrocystis lunula | |
| Sr2+ | - |
Lingulodinium polyedra | |
| Sr2+ | - |
Pyrocystis fusiformis | |
| Sr2+ | - |
Pyrocystis lunula | |
| Zn2+ | inhibits the light emission by dinoflagellate luciferase | Lingulodinium polyedra | |
| Zn2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Zn2+ | inhibits the light emission by dinoflagellate luciferase | Pyrocystis lunula | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| Ca2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| Mg2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
| Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Lingulodinium polyedra | |
| Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis lunula | |
| Mn2+ | increases the flash height and the total light emitted by dinoflagellate luciferase | Pyrocystis fusiformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Lingulodinium polyedra |
| 40000 | - |
SDS-PAGE | Pyrocystis lunula |
| 40000 | - |
SDS-PAGE | Pyrocystis fusiformis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | at 18 and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Lingulodinium polyedra |
| guanidine-HCl | at 18 and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis lunula |
| guanidine-HCl | at 18°C and 5°C guanidine-HCl in the concentration range from 2-6 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis fusiformis |
| urea | at 18 and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Lingulodinium polyedra |
| urea | at 18 and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis lunula |
| urea | at 18°C and 5°C urea in the concentration range from 2-8 M, denatures irreversibly the luciferase. Luciferin does not protect the luciferase against denaturation by this agent | Pyrocystis fusiformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lingulodinium polyedra | - |
formerly Gonyaulax polyedra | - |
| Pyrocystis fusiformis | - |
- |
- |
| Pyrocystis lunula | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Lingulodinium polyedra |
| ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Pyrocystis lunula |
| ammonium sulfate precipitation, Chelex column chromatography, hydroxylapatite column chromatography, and Ultrogel gel filtration | Pyrocystis fusiformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Lingulodinium polyedra |
| additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Pyrocystis lunula |
| additional information | - |
the crude extract has a specific activity of 0.000000258 at pH 8.0 and 20°C and the purified enzyme shows a specific activity of 0.00000348 quanta/min/mg at pH 8.0 and 20°C | Pyrocystis fusiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dinoflagellate luciferin + O2 | - |
Lingulodinium polyedra | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
| dinoflagellate luciferin + O2 | - |
Pyrocystis lunula | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
| dinoflagellate luciferin + O2 | - |
Pyrocystis fusiformis | oxidized dinoflagellate luciferin + H2O + hv | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Pyrocystis lunula |
| ? | x * 40000, SDS-PAGE | Pyrocystis fusiformis |
| ? | x * 30000, SDS-PAGE | Lingulodinium polyedra |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
about 20°C | Lingulodinium polyedra |
| 20 | - |
about 20°C | Pyrocystis lunula |
| 20 | - |
about 20°C | Pyrocystis fusiformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Lingulodinium polyedra |
| 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Pyrocystis lunula |
| 20 | 40 | at 20°C the luciferase is progressively inactivated after a 4 h incubation time at each pH, about 50% residual activity is observed at 30°C, the enzyme appears completely inactive at 40°C and pH 7.9 and shows about 10% residual activity at 40°C and pH 6.7 | Pyrocystis fusiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Lingulodinium polyedra |
| 7 | - |
- |
Pyrocystis lunula |
| 7 | - |
- |
Pyrocystis fusiformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Lingulodinium polyedra |
| 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Pyrocystis lunula |
| 6.7 | 8 | the crude enzyme extracts are more stable at pH 8.0 and 5°C in HEPES buffer than at pH 6.7 and 5°C | Pyrocystis fusiformis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lingulodinium polyedra | isoelectric focusing | - |
5.4 |
| Pyrocystis lunula | isoelectric focusing | - |
5.7 |
| Pyrocystis fusiformis | isoelectric focusing | - |
5.7 |
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