Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, relative activity remaining 2% | Neurospora crassa | |
cytochrome c | 0.04 mM, relative activity remaining 34% | Neurospora crassa | |
dithiothreitol | 1 mM, complete inhibition | Neurospora crassa | |
EDTA | 1 mM, relative activity remaining 95% | Neurospora crassa | |
GSH | 5 mM, relative activity remaining 28% | Neurospora crassa | |
iodoacetate | 1 mM, complete inhibition | Neurospora crassa | |
Nitro blue tetrazolium | 2.5 mM, relative activity remaining 10% | Neurospora crassa | |
Superoxide dismutase | complete inhibition | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | nitroethane | neutral form of substrate | Neurospora crassa | |
3.1 | - |
2-Nitropropane | anionic form of substrate | Neurospora crassa | |
3.1 | - |
propyl-2-nitronate | anionic form of substrate | Neurospora crassa | |
6 | - |
2-Nitropropane | anionic form of substrate | Neurospora crassa | |
6 | - |
ethyl nitronate | anionic form of substrate | Neurospora crassa | |
8.3 | - |
1-Nitropropane | anionic form of substrate | Neurospora crassa | |
8.3 | - |
propyl-1-nitronate | anionic form of substrate | Neurospora crassa | |
20 | - |
1-Nitropropane | neutral form of substrate | Neurospora crassa | |
23 | - |
nitroethane | neutral form of substrate | Neurospora crassa | |
33 | - |
2-Nitropropane | neutral form of substrate | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Neurospora crassa |
70000 | - |
gel filtration | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Neurospora crassa | - |
ATCC 10337 | - |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
to homogeneity | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10000 | - |
- |
Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxybutyl-2-nitronate + O2 | - |
Neurospora crassa | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
1-hydroxyethyl-2-nitronate + O2 | - |
Neurospora crassa | glycoaldehyde + HNO2 | - |
? | |
1-nitropentane + O2 | 3% of the activity with 2-nitropropane | Neurospora crassa | ? + HNO2 | - |
? | |
1-nitropropane + O2 | 21% of the activity with 2-nitropropane | Neurospora crassa | propionaldehyde + HNO2 | - |
? | |
2-hydroxypentyl-3-nitronate + O2 | - |
Neurospora crassa | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
2-nitroethanol + O2 | 13% of the activity with 2-nitropropane | Neurospora crassa | glycoaldehyde + HNO2 | - |
? | |
2-nitropropane + O2 | - |
Neurospora crassa | acetone + HNO2 | - |
? | |
3-nitro-2-butanol + O2 | 14% of the activity with 2-nitropropane | Neurospora crassa | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
3-nitro-2-pentanol + O2 | 20% of the activity with 2-nitropropane | Neurospora crassa | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
3-nitropropionic acid + O2 | 12% of the activity with 2-nitropropane | Neurospora crassa | ? | - |
? | |
cyclohexyl nitronate + O2 | - |
Neurospora crassa | ? + HNO2 | - |
? | |
ethyl nitronate + O2 | - |
Neurospora crassa | acetaldehyde + HNO2 | - |
? | |
additional information | anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds | Neurospora crassa | ? | - |
? | |
additional information | anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates | Neurospora crassa | ? | - |
? | |
nitrocyclohexane + O2 | 2% of the activity with 2-nitropropane | Neurospora crassa | ? + HNO2 | - |
? | |
nitroethane + O2 | 27% of the activity with 2-nitropropane | Neurospora crassa | acetaldehyde + HNO2 | - |
? | |
pentyl-1-nitronate + O2 | - |
Neurospora crassa | ? + HNO2 | - |
? | |
propionate-3-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
propyl-1-nitronate + O2 | - |
Neurospora crassa | propionaldehyde + HNO2 | - |
? | |
propyl-2-nitronate + O2 | - |
Neurospora crassa | acetone + HNO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
2-nitropropane dioxygenase | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
nitroethane | neutral form of substrate | Neurospora crassa | |
35 | - |
1-Nitropropane | neutral form of substrate | Neurospora crassa | |
160 | - |
2-Nitropropane | neutral form of substrate | Neurospora crassa | |
1300 | - |
1-Nitropropane | anionic form of substrate | Neurospora crassa | |
1300 | - |
propyl-1-nitronate | anionic form of substrate | Neurospora crassa | |
2000 | - |
nitroethane | anionic form of substrate | Neurospora crassa | |
2000 | - |
ethyl nitronate | anionic form of substrate | Neurospora crassa | |
6400 | - |
2-Nitropropane | anionic form of substrate | Neurospora crassa | |
6400 | - |
propyl-2-nitronate | anionic form of substrate | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | 2 mol of FMN per mol of subunit | Neurospora crassa | |
FMN | dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit | Neurospora crassa |