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Literature summary for 1.13.12.15 extracted from

  • Ranjith, N.K.; Ramana, C.h.V.; Sasikala, C.H.
    Purification and characterization of 3,4-dihydroxyphenylalanine oxidative deaminase from Rhodobacter sphaeroides OU5 (2008), Can. J. Microbiol., 54, 829-834.
    View publication on PubMed
Search for more literature for 1.13.12.15

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
 Cereibacter sphaeroides
NADH
-
 Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value for 3,4-dihydroxy-L-phenylalanine is 11.84 microM Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
 gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides OU5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxy-L-phenylalanine + O2
-
 Cereibacter sphaeroides 3,4-dihydroxyphenylpyruvate + NH3
-
 ?
3,4-dihydroxy-L-phenylalanine + O2
-
 Cereibacter sphaeroides OU5 3,4-dihydroxyphenylpyruvate + NH3
-
 ?
glycine + O2
-
 Cereibacter sphaeroides oxoacetic acid + NH3 30% of the activity with 3,4-dihydroxy-L-phenylalanine ?
glycine + O2
-
 Cereibacter sphaeroides OU5 oxoacetic acid + NH3 30% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-alanine + O2
-
 Cereibacter sphaeroides 2-oxo-propanoic acid + NH3 20% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-alanine + O2
-
 Cereibacter sphaeroides OU5 2-oxo-propanoic acid + NH3 20% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-glutamate + O2
-
 Cereibacter sphaeroides 2-oxopentanedioic acid + NH3 60% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-glutamate + O2
-
 Cereibacter sphaeroides OU5 2-oxopentanedioic acid + NH3 60% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-phenylalanine + H2O + O2
-
 Cereibacter sphaeroides 2-oxo-3-phenylpropanoic acid + NH3 60% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-phenylalanine + H2O + O2
-
 Cereibacter sphaeroides OU5 2-oxo-3-phenylpropanoic acid + NH3 60% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-tryptophan + O2
-
 Cereibacter sphaeroides 3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3 50% of the activity with 3,4-dihydroxy-L-phenylalanine ?
L-tyrosine + O2
-
 Cereibacter sphaeroides 3-(4-hydroxyphenyl)-2-oxopropanoic acid + NH3 80% of the activity with 3,4-dihydroxy-L-phenylalanine ?

Subunits

Subunits Comment Organism
pentamer five different subunits of 54000 Dalton, 43000 Dalton, 34000 Dalton, 25000 Dalton, and 22000 Dalton, SDS-PAGE Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 3,4-dihydroxy-L-phenylalanine pH 7.8, 30°C Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor required Cereibacter sphaeroides

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 IC50 value for NADH is 35 microM per l, IC50 value for 2-oxoglutarate is 25 microM per l Cereibacter sphaeroides additional information