Application | Comment | Organism |
---|---|---|
molecular biology | a multicolor BRET assay for the quantification of global DNA methylation is developed using CXXC-fused Oplophorus luciferase (CXXC-Oluc), a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc), BOBO-1, and BOBO-3. CXXC-Oluc recognized unmethylated CpG sites on genomic DNA to excite BOBO-1 DNA intercalating dye, whereas MBD-Fluc recognized methylated CpG sites on genomic DNA to excite BOBO-3 DNA intercalating dye. The emission intensities of BOBO-1 and BOBO-3 are simultaneously detected and depended on the unmethylated and methylated CpG contents of the genomic DNA. There is a significant negative correlation between the emission intensities of BOBO-1 and BOBO-3. Therefore, the global DNA methylation level can be quantified with this multicolor BRET assay using a single tube | Oplophorus sp. |
Organism | UniProt | Comment | Textmining |
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Oplophorus sp. | - |
- |
- |
Synonyms | Comment | Organism |
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CXXC-Oluc | - |
Oplophorus sp. |
Oplophorus luciferase | - |
Oplophorus sp. |