Protein Variants | Comment | Organism |
---|---|---|
H157N | over 3fold reduction in kcat/Km value | Azotobacter vinelandii |
Y159F | 3fold reduction in kcat/Km value. Fe coordination of cysteine is switched from thiolate only to bidentate (thiolate/amine) for the variant | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
3-Mercaptopropanoate | mutant Y159F, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
0.012 | - |
3-Mercaptopropanoate | mutant H157N, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
0.013 | - |
3-Mercaptopropanoate | wild-type, 20°C, pH not specified in the publication | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | - |
Azotobacter vinelandii | 3-sulfinopropanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
3-Mercaptopropanoate | mutant Y159F, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
0.092 | - |
3-Mercaptopropanoate | mutant H157N, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
0.45 | - |
3-Mercaptopropanoate | wild-type, 20°C, pH not specified in the publication | Azotobacter vinelandii |
General Information | Comment | Organism |
---|---|---|
physiological function | a network of hydrogen bonds connects residues H157-Y159 and Fe-bound ligands within the enzymatic Fe site. The hydroxyl group of Y159 hydrogen bonds to Fe-bound NO and, by extension, Fe-bound oxygen during native catalysis. This interaction alters both the NO binding affinity and rhombicity of the 3-mercaptopropanoate-bound iron-nitrosyl site | Azotobacter vinelandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.8 | - |
3-Mercaptopropanoate | mutant H157N, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
9 | - |
3-Mercaptopropanoate | mutant Y159F, 37°C, pH not specified in the publication | Azotobacter vinelandii | |
27 | - |
3-Mercaptopropanoate | wild-type, 20°C, pH not specified in the publication | Azotobacter vinelandii |