Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | enzyme has a specific requirement for L-cysteine (6.7 mM), required to restore full activity after dialysis or treatment with chelating agents | Achromobacter sp. |
General Stability | Organism |
---|---|
dialysis against phosphate buffer: complete loss of activity | Achromobacter sp. |
incubation with or dialysis against 0.001 mM 8-hydroxyquinoline and by (NH4)2SO4 fractionation: complete loss of activity | Achromobacter sp. |
L-cysteine: enzyme has a specific requirement for L-cysteine, 6.7 mM, required to restore full activity after dialysis or treatment with chelating agents | Achromobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pyridine-1,2-diol | - |
Achromobacter sp. | |
Pyridine-1,4-diol | - |
Achromobacter sp. | |
pyridine-2,3-diol | - |
Achromobacter sp. | |
pyridine-2,4-diol | - |
Achromobacter sp. | |
Pyridine-3,4-diol | - |
Achromobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ requirement | Achromobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
330000 | 340000 | gel filtration | Achromobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | - |
G2 and 2L | - |
Oxidation Stability | Organism |
---|---|
rapid loss of activity in air, purified enzyme, t1/2 is 2 h | Achromobacter sp. |
Purification (Comment) | Organism |
---|---|
partial | Achromobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.3 | - |
- |
Achromobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for at least several weeks, enzyme in crude extract | Achromobacter sp. |
4°C, several days, enzyme in crude extract | Achromobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxypyridine + O2 + H2O | - |
Achromobacter sp. | maleamate + formate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Achromobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, enzyme in crude extract stable | Achromobacter sp. |
65 | - |
10 min, enzyme in crude extract, 75% loss of activity | Achromobacter sp. |