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Literature summary for 1.13.11.9 extracted from

  • Gauthier, J.J.; Rittenberg, S.C.
    The metabolism of nicotinic acid. I. Purification and properties of 2,5-dihydroxypyridine oxygenase from Pseudomonas putida N-9 (1971), J. Biol. Chem., 246, 3737-3742.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas putida

General Stability

General Stability Organism
combination of dithiothreitol and FeSO4 is detrimental during long-term incubation at 0°C Pseudomonas putida
dithiothreitol stabilizes Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Pseudomonas putida
2,2'-dipyridyl
-
Pseudomonas putida
EDTA
-
Pseudomonas putida
H2O2
-
Pseudomonas putida
iodoacetamide
-
Pseudomonas putida
KCN
-
Pseudomonas putida
N-ethylmaleimide
-
Pseudomonas putida
p-chloromercuribenzoate
-
Pseudomonas putida
Tiron i.e. 4,5-dihydroxy-1,3-benzene disulfonic acid Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe2+ requirement Pseudomonas putida
Iron enzyme contains loosly bound Fe3+ Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
x * 39500, SDS-PAGE Pseudomonas putida
242000
-
sucrose density gradient centrifugation (+ dithiothreitol) Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-dihydroxypyridine + O2 + H2O Pseudomonas putida nicotinic acid catabolism maleamate + formate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.3
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-40°C, 3 months, 20% loss of activity Pseudomonas putida
0°C, 20 mM sodium phosphate, pH 7.5, half-life: 2-3 days, addition of dithiothreitol extends half-life to about 2 weeks Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxypyridine + O2 + H2O strictly specific for 2,5-dihydroxypyridine Pseudomonas putida maleamate + formate
-
?
2,5-dihydroxypyridine + O2 + H2O nicotinic acid catabolism Pseudomonas putida maleamate + formate
-
?

Subunits

Subunits Comment Organism
? x * 39500, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas putida