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Literature summary for 1.13.11.82 extracted from
- Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts beta-apo-8'-carotenal to beta-apo-13-carotenone (2012), Biotechnol. Lett., 34, 1851-1856 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Novosphingobium aromaticivorans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H170A | the mutant has 0.08% iron content and 2.3% activity compared to the wild type enzyme | Novosphingobium aromaticivorans |
| H219A | the mutant has no iron content and 1.1% activity compared to the wild type enzyme | Novosphingobium aromaticivorans |
| H284A | the mutant has 0.14% iron content and 2% activity compared to the wild type enzyme | Novosphingobium aromaticivorans |
| H460A | the inactive mutant has no iron content | Novosphingobium aromaticivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
8'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans |  |
| 0.037 | - |
4'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
| 0.203 | - |
gamma-carotene | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 0.01 mM Fe2+ strongly enhances the enzyme activity | Novosphingobium aromaticivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
gel filtration | Novosphingobium aromaticivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8'-apo-beta-carotenal + O2 | Novosphingobium aromaticivorans | - |
13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
| 8'-apo-beta-carotenal + O2 | Novosphingobium aromaticivorans DSM 12444 | - |
13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Novosphingobium aromaticivorans | - |
- |
- |
| Novosphingobium aromaticivorans DSM 12444 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap affinity column chromatography | Novosphingobium aromaticivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4'-apo-beta-carotenal + O2 | - |
Novosphingobium aromaticivorans | ? | - |
? | |
| 4'-apo-beta-carotenal + O2 | - |
Novosphingobium aromaticivorans DSM 12444 | ? | - |
? | |
| 8'-apo-beta-carotenal + O2 | - |
Novosphingobium aromaticivorans | 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
| 8'-apo-beta-carotenal + O2 | best substrate | Novosphingobium aromaticivorans | 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
| 8'-apo-beta-carotenal + O2 | - |
Novosphingobium aromaticivorans DSM 12444 | 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
| 8'-apo-beta-carotenal + O2 | best substrate | Novosphingobium aromaticivorans DSM 12444 | 13-apo-beta-carotenone + 2,6-dimethyldeca-2,4,6,8-tetraenedial | - |
? | |
| gamma-carotene + O2 | - |
Novosphingobium aromaticivorans | ? | - |
? | |
| gamma-carotene + O2 | - |
Novosphingobium aromaticivorans DSM 12444 | ? | - |
? | |
| additional information | no activity with alpha-carotene, beta-carotene, beta-cryptoxanthin, zeaxanthin, lutein, beta-apo-12'-carotenal, and lycopene | Novosphingobium aromaticivorans | ? | - |
- |
|
| additional information | no activity with alpha-carotene, beta-carotene, beta-cryptoxanthin, zeaxanthin, lutein, beta-apo-12'-carotenal, and lycopene | Novosphingobium aromaticivorans DSM 12444 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE | Novosphingobium aromaticivorans |
| monomer | 1 * 52130, calculated from amino acid sequence | Novosphingobium aromaticivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| apo-carotenoid 13,14-dioxygenase | - |
Novosphingobium aromaticivorans |
| NACOX1 | - |
Novosphingobium aromaticivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Novosphingobium aromaticivorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | 60% or more activity between 35 and 50°C, about 25% activity at 55°C | Novosphingobium aromaticivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | the half-lives of the enzyme at 40, 45, 50, 55 and 60°C are 23.4, 15.3, 8.2, 3.8, and 0.6 h, respectively | Novosphingobium aromaticivorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
gamma-carotene | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
| 0.14 | - |
4'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
| 0.417 | - |
8'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Novosphingobium aromaticivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | more than 60% activity between pH 7.0 and 9.0 | Novosphingobium aromaticivorans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
gamma-carotene | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
| 3.78 | - |
4'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
| 19.57 | - |
8'-apo-beta-carotenal | at pH 8.0 and 45°C | Novosphingobium aromaticivorans | |
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