Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 79% residual activity | Comamonas testosteroni | |
Cd2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
Co2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
Cu2+ | 2 mM, 2.9% residual activity | Comamonas testosteroni | |
EDTA | 2 mM, 50% residual activity | Comamonas testosteroni | |
Fe2+ | 2 mM, 57% residual activity. Not inhibitory in presence of ascorbate | Comamonas testosteroni | |
H2O2 | 2 mM, complete loss of activity | Comamonas testosteroni | |
Mg2+ | 2 mM, 91% residual activity | Comamonas testosteroni | |
Mn2+ | 2 mM, 31% residual activity | Comamonas testosteroni | |
Ni2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni | |
Zn2+ | 2 mM abolishes enzyme activity completely | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
2-amino-5-chlorophenol | pH 8.0, 22°C | Comamonas testosteroni | |
0.00089 | - |
2-Aminophenol | pH 8.0, 22°C | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
38000 | - |
2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
130000 | - |
gel filtration | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q6J1Z5 | subunit alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.7 | - |
pH 8.0, 22°C | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-5-chlorophenol + O2 | 68% of the activity with 2-aminophenol | Comamonas testosteroni | 2-amino-5-chloromuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | - |
Comamonas testosteroni | ? | - |
? | |
catechol + O2 | 5% of the activity with 2-aminophenol | Comamonas testosteroni | ? | - |
? | |
additional information | no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol | Comamonas testosteroni | ? | - |
? | |
protocatechuate + O2 | 33% of the activity with 2-aminophenol | Comamonas testosteroni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
CnbcA | - |
Comamonas testosteroni |