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Literature summary for 1.13.11.63 extracted from

  • Kowatz, T.; Babino, D.; Kiser, P.; Palczewski, K.; von Lintig, J.
    Characterization of human beta,beta-carotene-15,15'-monooxygenase (BCMO1) as a soluble monomeric enzyme (2013), Arch. Biochem. Biophys., 539, 214-222.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.63

Cloned(Commentary)

Cloned (Comment) Organism
gene BCMO1, expression of enzyme with C-terminal C-terminal TEV-, His6- and 1D4-tags in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate at 0.245% Homo sapiens
hexaethylene glycol monooctyl ether at 0.195-0.78% Homo sapiens
additional information addition of detergents fails to increase BCMO1 enzymatic activity, but short chain aliphatic detergents such as C8E4 and C8E6 decrease enzymatic activity probably by interacting with the substrate binding site Homo sapiens
n-octyl-beta-D-thioglucopyranoside at 0.56% Homo sapiens
tetraethylene glycol monooctyl ether at 0.125-0.5% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, overview Homo sapiens
0.0137 0.0148 beta-carotene recombinant enzyme, pH 7.5, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-
cytosol
-
 Homo sapiens 5829
-
additional information determination of BCMO1 subcellular localization in mouse liver, overview Mus musculus
-
-
soluble BCMO1 is soluble and its enzymatic activity is not dependent on a membrane-like environment Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme is a non-heme iron oxygenase Mus musculus
Fe2+ the enzyme is a non-heme iron oxygenase Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Mus musculus
-
 2 all-trans-retinal
-
 ?
beta-carotene + O2 Homo sapiens
-
 2 all-trans-retinal
-
 ?
beta-carotene + O2 Mus musculus C57/BL6 / 129 Sv
-
 2 all-trans-retinal
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAY6 gene Bcmo1
-
Mus musculus Q9JJS6 gene Bcmo1
-
Mus musculus C57/BL6 / 129 Sv Q9JJS6 gene Bcmo1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from Stellate cell cytosol Mus musculus
recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags from Spodoptera frugiperda Sf9 cells by Co2+ affinity chromatography and gel filtration in absence of detergent Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Mus musculus
-
stellate cell high enzyme expression Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Mus musculus 2 all-trans-retinal
-
 ?
beta-carotene + O2
-
 Homo sapiens 2 all-trans-retinal
-
 ?
beta-carotene + O2
-
 Mus musculus C57/BL6 / 129 Sv 2 all-trans-retinal
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Mus musculus
monomer 1 x 65200, recombinant enzyme with C-terminal TEV-, His6- and 1D4-tags, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Bcmo1
-
 Mus musculus
Bcmo1
-
 Homo sapiens
beta,beta-carotene-15,15'-monooxygenase
-
 Mus musculus
beta,beta-carotene-15,15'-monooxygenase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5 8.5 beta-carotene recombinant enzyme, pH 7.5, temperature not specified in the publication Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information recombinant BCMO1 does not require cofactors Homo sapiens