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Literature summary for 1.13.11.63 extracted from

  • Kim, Y.S.; Park, C.S.; Oh, D.K.
    Hydrophobicity of residue 108 specifically affects the affinity of human beta-carotene 15,15'-monooxygenase for substrates with two ionone rings (2010), Biotechnol. Lett., 32, 847-853.
    View publication on PubMed
Search for more literature for 1.13.11.63

Cloned(Commentary)

Cloned (Comment) Organism
BCMO gene, DNA and mino acid sequence determination and analysis, expression in Escherichia coli strain ER2566 Homo sapiens

Protein Variants

Protein Variants Comment Organism
K108F site-directed mutagenesis, the mutant shows highly decreased affinity for substrates with ionone rings at both ends, such as alpha-carotene, beta-carotene, and beta-cryptoxanthin, and a 7.2fold increased Km for beta-carotene compared to the wild-type enzyme. But the mutation has little effect on the affinity of the enzyme for substrates with one ionone ring and one open-chain end, such as beta-apo-4'-carotenal and beta-apo-8'-carotenal Homo sapiens
K108L site-directed mutagenesis, the mutant shows highly decreased affinity for substrates with ionone rings at both ends, such as alpha-carotene, beta-carotene, and beta-cryptoxanthin, and a 2.9fold increased Km for beta-carotene compared to the wild-type enzyme. But the mutation has little effect on the affinity of the enzyme for substrates with one ionone ring and one open-chain end, such as beta-apo-4'-carotenal and beta-apo-8'-carotenal Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics of wild-type enzyme and mutants K108L and K108F with beta-carotene and beta-apo-8'-carotenal, overview Homo sapiens
0.03
-
 beta-carotene pH 8.0, 37°C, recombinant wild-type enzyme Homo sapiens
0.088
-
 beta-carotene pH 8.0, 37°C, recombinant mutant K108L Homo sapiens
0.215
-
 beta-carotene pH 8.0, 37°C, recombinant mutant K108F Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + O2 Homo sapiens
-
 2 retinal
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAY6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by affinity and anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + O2
-
 Homo sapiens 2 retinal
-
 ?
beta-carotene + O2 substrate binding structure, overview Homo sapiens 2 retinal
-
 ?
beta-cryptoxanthin + O2
-
 Homo sapiens ?
-
 ?
additional information BCMO is also active with alpha-carotene, beta-apo-8'-carotenal, and beta-apo-4'-carotenal. The hydrophobicity of residue 108 specifically affects the affinity of beta-carotene 15,15'-monooxygenase for substrates with two ionone rings. Residue 108 may be related to the indirect interaction with the second ionone ring of the substrates with two ionone rings, comparison with apo-carotenoid 15,15'-oxygenase, EC 1.14.99.41, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More BCMO three-dimensional structure, with entrance of active tunnel and the hydrophobic patch, including Pro101, Cys102, Ile105, Phe106, Lys108, Leu258, Thr262, and Tyr264, modelling, overview Homo sapiens

Synonyms

Synonyms Comment Organism
BCMO
-
 Homo sapiens
beta-carotene 15,15-monooxygenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0395
-
 beta-carotene pH 8.0, 37°C, recombinant mutant K108L Homo sapiens
0.04
-
 beta-carotene pH 8.0, 37°C, recombinant mutant K108F Homo sapiens
0.046
-
 beta-carotene pH 8.0, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens