Application | Comment | Organism |
---|---|---|
analysis | BCMO function in mammalian cells is analyzed by a retinoic acid receptor assay, which responds to the metabolic conversion of beta-carotene to retinoic acid in vivo, this tool can be used to screen more active BCMO for the industrial and pharmacological purpose of retinal production from beta-carotene | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into a Flag fusion-modified mammalian expression vector pcDNA3 and into the bacterial vectors pET-15b and pGEX-4T2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T381L | mutant, shows 1.5fold higher catalytic efficiency for beta-carotene and 1.7fold higher retinal production than the wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
beta-carotene | T381L mutant | Homo sapiens | |
0.031 | - |
beta-carotene | wild-type | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
theoretical | Homo sapiens |
65000 | - |
determined by SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | Homo sapiens | - |
retinal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAY6 | - |
- |
Purification (Comment) | Organism |
---|---|
using a HiTrap Chelating HP column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-704 cell | human kidney cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + O2 | - |
Homo sapiens | retinal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCMO | - |
Homo sapiens |
beta-carotene-15,15'-monooxygenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Homo sapiens |