Crystallization (Comment) | Organism |
---|---|
first vapor diffusion with sitting drop method starting from protein concentration of 10 mg/ml in 32% (NH4)2SO4, 0.1 M sodium acetate, 10 mM 2-mercaptoethanol, pH 5, subsequently, crystals can be obtained by seeding starting from fragments of first crystallization experiments using 40% (NH4)2SO4, Tris-HCl, 40 mM MgCl2, 3% MPD, pH 8 as precipitant | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE, nondenaturating PAGE resolves two components, purified by FPLC on protein PakGlass DEAE-4 PW column, one component is N-terminally truncated annexin IV | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-3-hydroxybenzoic acid + O2 | Bos taurus | - |
2,3-pyridinedicarboxylic acid + H2O | intermediate 2-amino-3-carboxymuconic acid semialdehyde | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q0VCA8 | - |
- |
Purification (Comment) | Organism |
---|---|
bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-3-hydroxybenzoic acid + O2 | - |
Bos taurus | 2,3-pyridinedicarboxylic acid + H2O | intermediate 2-amino-3-carboxymuconic acid semialdehyde | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE, 286 amino acids, 2 domains that represent the dimers of the prokaryote enzyme structure which is also conserved in simple eukaryotes | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxyanthranilate 3,4-dioxygenase | - |
Bos taurus |
3HAO | - |
Bos taurus |