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Literature summary for 1.13.11.56 extracted from

  • Kuhm, A.E.; Stolz, A.; Ngai, K.L.; Knackmuss, H.J.
    Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids (1991), J. Bacteriol., 173, 3795-3802.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.56

General Stability

General Stability Organism
enzyme in crude extract is stable in Na-K phosphate buffer, pH 7.5, after 1 week of storage (2.4 mg of protein per ml) at 4°C, 100% of the original activity is recovered Sphingobium xenophagum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 1,2-dihydroxynaphthalene
-
 Sphingobium xenophagum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for maximal activity Sphingobium xenophagum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 8 * 33000, SDS-PAGE Sphingobium xenophagum
290000
-
 gel filtration Sphingobium xenophagum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dihydroxynaphthalene + O2 Sphingobium xenophagum induced in presence of naphthalene-2-sulfonic acid 2-hydroxychromene-2-carboxylate
-
 ?
1,2-dihydroxynaphthalene + O2 Sphingobium xenophagum BN6 induced in presence of naphthalene-2-sulfonic acid 2-hydroxychromene-2-carboxylate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated Sphingobium xenophagum
Ethanol storage for 1 week at 4°C in the presence of 10% (v/v) ethanol or acetone, the enzyme in crude extract is completely inactivated Sphingobium xenophagum

Organism

Organism UniProt Comment Textmining
Sphingobium xenophagum
-
 BN6
-
Sphingobium xenophagum BN6
-
 BN6
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium xenophagum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.36
-
-
 Sphingobium xenophagum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,5-trihydroxynaphthalene + O2
-
 Sphingobium xenophagum ?
-
 ?
1,2,6-trihydroxynaphthalene + O2
-
 Sphingobium xenophagum ?
-
 ?
1,2,7-trihydroxynaphthalene + O2
-
 Sphingobium xenophagum ?
-
 ?
1,2-dihydroxynaphthalene + O2 induced in presence of naphthalene-2-sulfonic acid Sphingobium xenophagum 2-hydroxychromene-2-carboxylate
-
 ?
1,2-dihydroxynaphthalene + O2 induced in presence of naphthalene-2-sulfonic acid Sphingobium xenophagum BN6 2-hydroxychromene-2-carboxylate
-
 ?
1,2-dihydroxynaphthalene + O2
-
 Sphingobium xenophagum 2-hydroxy-2H-chromene-2-carboxylate
-
 ?
2,3-dihydroxybiphenyl + O2
-
 Sphingobium xenophagum ?
-
 ?
2,3-dihydroxybiphenyl + O2
-
 Sphingobium xenophagum BN6 ?
-
 ?
3,4-dihydroxybiphenyl + O2
-
 Sphingobium xenophagum ?
-
 ?
3-methylcatechol + O2
-
 Sphingobium xenophagum ?
-
 ?
3-methylcatechol + O2
-
 Sphingobium xenophagum BN6 ?
-
 ?
4-methylcatechol + O2
-
 Sphingobium xenophagum ?
-
 ?
4-methylcatechol + O2
-
 Sphingobium xenophagum BN6 ?
-
 ?
catechol + O2
-
 Sphingobium xenophagum ?
-
 ?
catechol + O2
-
 Sphingobium xenophagum BN6 ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 33000, SDS-PAGE Sphingobium xenophagum

Synonyms

Synonyms Comment Organism
1,2-DHN dioxygenase
-
 Sphingobium xenophagum
DHNDO
-
 Sphingobium xenophagum

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 the enzyme is more stable at slightly alkaline pH values and better preserved in Na-K phosphate buffer than in Tris-HCI or glycine-NaOH Sphingobium xenophagum