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Literature summary for 1.13.11.56 extracted from

  • Patel, T.R.; Barnsley, E.A.
    Naphthalene metabolism by pseudomonads: purification and properties of 1,2-dihydroxynaphthalene oxygenase (1980), J. Bacteriol., 143, 668-673.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.56

General Stability

General Stability Organism
the enzyme is inactivated slowly on standing, and inactivation is accelerated by dilution with aerated buffers and by H2O2. The inactive enzyme is reactivated by anaerobic incubation with Fe(SO4) and ferrous ammonium sulfate. Thiol reagents and acetone, ethanol, or glycerol decrease the rate of loss of activity Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
1,2,3-trihydroxybenzene 0.0005 mM, 76% inhibition Pseudomonas putida
2,2'-dipyridyl
-
 Pseudomonas putida
2,3-dihydroxynaphthalene 0.0005 mM, 44% inhibition Pseudomonas putida
2,7-dihydroxynaphthalene 0.0005 mM, 64% inhibition Pseudomonas putida
3,4-dihydroxybenzoic acid 0.0005 mM, 20% inhibition Pseudomonas putida
3-methylcatechol 0.0005 mM, 76% inhibition, competitive inhibitor Pseudomonas putida
4-methylcatechol 0.0005 mM, 36% inhibition, competitive inhibitor Pseudomonas putida
8-hydroxyquinoline
-
 Pseudomonas putida
bathophenanthroline sulfonate
-
 Pseudomonas putida
Co2+
-
 Pseudomonas putida
Cu2+
-
 Pseudomonas putida
Fe3+
-
 Pseudomonas putida
Hg2+
-
 Pseudomonas putida
o-phenanthroline
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 3-methylcatechol pH 6.2, 25°C Pseudomonas putida
0.27
-
 O2 pH 6.2, 25°C Pseudomonas putida
0.28
-
 1,2-dihydroxynaphthalene pH 6.2, 25°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required. Other metal ions (Fe3+, C02+, Ca2+, Cu2+, Hg2+, Ni2+, Zn2+, Mg2+) could not replace Fe2+ Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE Pseudomonas putida
275000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 NCIB 9816
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:naphthalene-grown cell
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxynaphthalene + O2 2-hydroxychromene-2-carboxylic acid may result from cycization of a ring fission product before release from the enzyme Pseudomonas putida 2-hydroxy-2H-chromene-2-carboxylate
-
 ?
3-methylcatechol + O2 5% of the activity with 1,2-dihydroxynaphthalene. The product 2-hydroxy-6-oxoheptadienoic acid is not structurally analogous to 2-hydroxychromene-2-carboxylic acid Pseudomonas putida 2-hydroxy-6-oxohepta-2,4-dienoic acid
-
 ?
4-methylcatechol + O2 2% of the activity with 1,2-dihydroxynaphthalene Pseudomonas putida ?
-
 ?
additional information purified enzyme shows no activity with catechol Pseudomonas putida ?
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
1,2-dihydroxynaphthalene oxygenase
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudomonas putida