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Literature summary for 1.13.11.55 extracted from

  • Urich, T.; Kroke, A.; Bauer, C.; Seyfarth, K.; Reuff, M.; Kletzin, A.
    Identification of core active site residues of the sulfur oxygenase reductase from Acidianus ambivalens by site-directed mutagenesis (2005), FEMS Microbiol. Lett., 248, 171-176.
    View publication on PubMed
Search for more literature for 1.13.11.55

Application

Application Comment Organism
degradation initial enzyme in the sulfur-oxidation pathway Acidianus ambivalens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Acidianus ambivalens

Protein Variants

Protein Variants Comment Organism
C101/104A enzyme with decreased specific activity Acidianus ambivalens
C101/104S enzyme with decreased specific activity Acidianus ambivalens
C101A enzyme with decreased specific activity Acidianus ambivalens
C101A iron content is 49% of that of the recombinant wild-type enzyme, oxygenase activity is 12.8% of the activity of recombinant wild-type enzyme, reductase activity is 10.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C101A/C104A iron content is 124% of that of the recombinant wild-type enzyme, oxygenase activity is 9.7% of the activity of recombinant wild-type enzyme, reductase activity is 15.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C101S enzyme with decreased specific activity and a proportional decrease in iron content Acidianus ambivalens
C101S mutant enzyme contains no iron, oxygenase activity is 1.04% of the activity of recombinant wild-type enzyme, reductase activity is 0.5% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C101S/C104S iron content is 89% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 27.9% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C104A enzyme with decreased specific activity Acidianus ambivalens
C104A iron content is 42% of that of the recombinant wild-type enzyme, oxygenase activity is 11.8% of the activity of recombinant wild-type enzyme, reductase activity is 5.5% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C104S enzyme with decreased specific activity Acidianus ambivalens
C104S iron content is 67% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 14.3% of the activity of recombinant wild-type enzyme Acidianus ambivalens
C31A inactive enzyme Acidianus ambivalens
C31A inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme Acidianus ambivalens
C31S inactive enzyme Acidianus ambivalens
C31S inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme Acidianus ambivalens
E114A inactive enzyme with no iron incorporated Acidianus ambivalens
E114A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens
E114D enzyme with 1% of wild type activity Acidianus ambivalens
E114D iron content is 4.4% of wild-type value, sulfur-oxidizing and sulfur-reducing activity is about 1% of the activity of activity of the recombinant wild-type enzyme Acidianus ambivalens
H86A inactive enzyme with no iron incorporated Acidianus ambivalens
H86A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens
H90A inactive enzyme with no iron incorporated Acidianus ambivalens
H90A mutation results in inactive enzyme with no measurable iron found Acidianus ambivalens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron content: 0.45 mol per mol subunit for recombinant wild-type enzyme, below 0.1 mol per mol subunit for mutant enzyme H86A, below 0.02 mol per mol subunit for mutant enzyme H90A, below 0.01 mol per mol subunit for mutant enzyme E114A, 0.02 mol per mol subunit for mutant enzyme E114D, 0.47 mol per mol subunit for mutant enzyme C31A, 0.42 mol per mol subunit for mutant enzyme C31S, 0.22 mol per mol subunit for mutant enzyme C101A, below 0.03 mol per mol subunit for mutant enzyme C101S, 0.19 mol per mol subunit for mutant enzyme C104A, 0.3 mol per mol subunit for mutant enzyme C104S, 0.56 mol per mol subunit for mutant enzyme C101A/C104A, 0.4 mol per mol subunit for mutant enzyme C101S/C104S Acidianus ambivalens
Fe2+ mononucler non-heme iron site Acidianus ambivalens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfur + H2O + O2 Acidianus ambivalens initial enzyme in the sulfur oxidation pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acidianus ambivalens
-
-
-
Acidianus ambivalens P29082
-
-

Purification (Commentary)

Purification (Comment) Organism
one-step procedure over Strep-Tactin columns Acidianus ambivalens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 mutant C101S, U/mg, reductase reaction Acidianus ambivalens
0.03
-
 mutant E114D, U/mg, reductase reaction Acidianus ambivalens
0.11
-
 mutant E114D, U/mg, oxygenase reaction Acidianus ambivalens
0.12
-
 mutant C101S, U/mg, oxygenase reaction Acidianus ambivalens
0.23
-
 mutant C104A, U/mg, reductase reaction Acidianus ambivalens
0.43
-
 mutant C101A, U/mg, reductase reaction Acidianus ambivalens
0.6
-
 mutant C104S, U/mg, reductase reaction Acidianus ambivalens
0.64
-
 mutant C101/104A, U/mg, reductase reaction Acidianus ambivalens
1.12
-
 mutant C101/104A, U/mg, oxygenase reaction Acidianus ambivalens
1.17
-
 mutant C101/104S, U/mg, reductase reaction Acidianus ambivalens
1.35
-
 mutant C104A, U/mg, oxygenase reaction Acidianus ambivalens
1.47
-
 mutant C101, U/mg, oxygenase reaction Acidianus ambivalens
2.28
-
 mutant C104S, U/mg, oxygenase reaction Acidianus ambivalens
2.29
-
 mutant C101/104S, U/mg, oxygenase reaction Acidianus ambivalens
4.19
-
 wild type, U/mg, reductase reaction Acidianus ambivalens
11.52
-
 wild type, U/mg, oxygenase reaction Acidianus ambivalens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfur + H2O + O2 initial enzyme in the sulfur oxidation pathway Acidianus ambivalens ?
-
 ?

Synonyms

Synonyms Comment Organism
SOR
-
 Acidianus ambivalens
sulfur oxygenase reductase
-
 Acidianus ambivalens