Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)pLysS | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H98S | mutation results in the formation of a stable soluble protein that while structurally different from ARD-Ni+2 shows a high degree of similarity to the ARD'(Fe) enzyme | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | Ni2+ can be conservatively replaced by Mn2 +or Co2+, giving rise to ARD activity (CO production) | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 | - |
Mus musculus | 4-methylthio-2-ketobutyrate + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x-ray crystallography | Mus musculus |