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Literature summary for 1.13.11.52 extracted from

  • Sono, M.
    Enzyme kinetic and spectroscopic studies of inhibitor and effector interactions with indoleamine 2,3-dioxygenase. 2. Evidence for the existence of another binding site in the enzyme for indole derivative effectors (1989), Biochemistry, 28, 5400-5407.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-indoleethanol 1 mM, enhances activity by 87% Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
3-indoleethanol lowers Km value for D-tryptophan by 25% at pH 7, enhances Vmax by 40-60% Oryctolagus cuniculus
azide
-
Oryctolagus cuniculus
CO
-
Oryctolagus cuniculus
cyanide heme ligand, competitive for the ferric enzyme Oryctolagus cuniculus
indole lowers Km value for D-tryptophan by 60% at pH 7, lowers Vmax by 12-24%, at 1 mM lowers activity by 13% Oryctolagus cuniculus
additional information 3-indoleethanol and indole affect the L-tryptophan affinity of the ferrous enzyme, indole enhances affinity of enzyme for cyanide and azide and norharman, L-tryptophan lowers norharman affinity for the ferrous dioxygenase Oryctolagus cuniculus
NO
-
Oryctolagus cuniculus
norharman can bind to the ferric and ferrous enzyme to from a quarternary complex Oryctolagus cuniculus
O2
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
L-tryptophan pH 6.0 Oryctolagus cuniculus
0.66
-
D-tryptophan pH 7.0, 1 mM indole Oryctolagus cuniculus
1.3
-
D-tryptophan pH 7.0, 1 mM 3-indoleethanol Oryctolagus cuniculus
1.7
-
D-tryptophan pH 7.0 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-D-kynurenine
-
?
L-tryptophan + O2 Oryctolagus cuniculus
-
N-formyl-L-kynurenine
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Oxidation Stability

Oxidation Stability Organism
highly autoxidizable, requires superoxide anion or a reductant such as ascorbic acid and methylene blue as cofactors Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-D-kynurenine
-
?
L-tryptophan + O2
-
Oryctolagus cuniculus N-formyl-L-kynurenine
-
?

Cofactor

Cofactor Comment Organism Structure
additional information ascorbic acid in combination with methylene blue or toluidine blue Oryctolagus cuniculus
additional information superoxide anion in combination with methylene blue or toluidine blue Oryctolagus cuniculus