Cloned (Comment) | Organism |
---|---|
gene SlNCED1, real-time quantitative reverse transcription, phylogenetic tree | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fruit-specific suppression of gene SlNCED1, avoid the pleiotropic phenotypes associated with abscisic acid deficiency, using fruit-specific E8 promoter in RNAi constructs, abscisic acid accumulation and SlNCED1 transcript levels in the transgenic fruit are downregulated to between 20% and 50% of the levels measured in the control fruit. Significant reduction in 9-cis-epoxycarotenoid dioxygenase activity leads to a downregulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase, pectin methyl esterase, beta-galactosidase precursor mRNA, xyloglucan endotransglycosylase, endo-1,4-beta cellulose, and expansin resulting in an increased accumulation of pectin during ripening and thus to significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45% | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
gene SlNCED1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
General Information | Comment | Organism |
---|---|---|
malfunction | suppression of 9-cis-epoxycarotenoid dioxygenase alters fruit texture in transgenic tomato. Significant reduction in 9-cis-epoxycarotenoid dioxygenase activity leads to a downregulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase, pectin methyl esterase, beta-galactosidase precursor mRNA, xyloglucan endotransglycosylase, endo-1,4-beta cellulose, and expansin resulting in an increased accumulation of pectin during ripening and thus to significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%, phenotypes, overview | Solanum lycopersicum |
metabolism | 9-cis-epoxycarotenoid dioxygenase is a key enzyme in abscisic acid biosynthesis | Solanum lycopersicum |