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Literature summary for 1.13.11.50 extracted from

  • Straganz, G.D.; Egger, S.; Aquino, G.; DAuria, S.; Nidetzky, B.
    Exploring the cupin-type metal-coordinating signature of acetylacetone dioxygenase Dke1 with site-directed mutagenesis: Catalytic reaction profile and Fe2+ binding stability of Glu-69->Gln mutant (2006), J. Mol. Catal. B, 39, 171-178.
No PubMed abstract available
Search for more literature for 1.13.11.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter johnsonii

Protein Variants

Protein Variants Comment Organism
E69Q lower thermal stability of beta-sheet secondary structure, half catalytic center activity and remarkably silent difference in apparent substrate binding compared to the wild type enzyme Acinetobacter johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 Pentane-2,4-dione wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C Acinetobacter johnsonii
0.0065
-
 Pentane-2,4-dione mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme, 1 molecule per subunit Acinetobacter johnsonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 SDS-PAGE, Strep-tagged full length enzyme Acinetobacter johnsonii

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity column chromatography Acinetobacter johnsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-trifluoro-pentane-2,4-dione + O2
-
 Acinetobacter johnsonii ?
-
 ?
pentane-2,4-dione + O2
-
 Acinetobacter johnsonii acetate + 2-oxopropanal
-
 ?

Synonyms

Synonyms Comment Organism
acetylacetone dioxygenase
-
 Acinetobacter johnsonii
Dke1
-
 Acinetobacter johnsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0043
-
 1,1,1-trifluoro-pentane-2,4-dione native enzyme, using 0.09 mM 1,1,1-trifluoro-pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C Acinetobacter johnsonii
3.6
-
 Pentane-2,4-dione mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C Acinetobacter johnsonii
6.6
-
 Pentane-2,4-dione wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C Acinetobacter johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Acinetobacter johnsonii