Literature summary for 1.13.11.50 extracted from

Straganz, G.; Slavica, A.; Hofer, H.; Mandl, U.; Steiner, W.; Nidetzky, B.
Integrated approach for production of recombinant acetylacetone dioxygenase from Acinetobacter johnsonii (2005), Biocatal. Biotransform., 23, 261-269.

No PubMed abstract available

Search for more literature for 1.13.11.50

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21(DE3)	Acinetobacter johnsonii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	Pentane-2,4-dione	Strep-tagged enzyme, at pH 7.5	Acinetobacter johnsonii
0.008	-	Pentane-2,4-dione	recombinant enzyme, at pH 7.5	Acinetobacter johnsonii
0.009	-	Pentane-2,4-dione	native enzyme, at pH 7.5	Acinetobacter johnsonii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	essential for activity	Acinetobacter johnsonii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
16600	-	4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS	Acinetobacter johnsonii
16607	-	4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS	Acinetobacter johnsonii
66400	-	SDS-PAGE	Acinetobacter johnsonii
66430	-	MALDI-TOF MS	Acinetobacter johnsonii

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter johnsonii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose Q fast flow column chromatography Superdex 200 gel filtration, Resource Q column chromatography, Nap-5 column gel filtration, and Phenyl Sepharose HP chromatography	Acinetobacter johnsonii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pentane-2,4-dione + O2	-	Acinetobacter johnsonii	acetate + 2-oxopropanal	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS	Acinetobacter johnsonii

Synonyms

Synonyms	Comment	Organism
acetylacetone dioxygenase	-	Acinetobacter johnsonii
Dke1	-	Acinetobacter johnsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.6	-	Pentane-2,4-dione	Strep-tagged enzyme	Acinetobacter johnsonii
7	-	Pentane-2,4-dione	recombinant enzyme	Acinetobacter johnsonii
8.5	-	Pentane-2,4-dione	native enzyme	Acinetobacter johnsonii

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Release 2023.1 (January 2023)