Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Acinetobacter johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
Pentane-2,4-dione | Strep-tagged enzyme, at pH 7.5 | Acinetobacter johnsonii | |
0.008 | - |
Pentane-2,4-dione | recombinant enzyme, at pH 7.5 | Acinetobacter johnsonii | |
0.009 | - |
Pentane-2,4-dione | native enzyme, at pH 7.5 | Acinetobacter johnsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | essential for activity | Acinetobacter johnsonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16600 | - |
4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS | Acinetobacter johnsonii |
16607 | - |
4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS | Acinetobacter johnsonii |
66400 | - |
SDS-PAGE | Acinetobacter johnsonii |
66430 | - |
MALDI-TOF MS | Acinetobacter johnsonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose Q fast flow column chromatography Superdex 200 gel filtration, Resource Q column chromatography, Nap-5 column gel filtration, and Phenyl Sepharose HP chromatography | Acinetobacter johnsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 16600, SDS-PAGE, 4 * 16607, MALDI-TOF MS | Acinetobacter johnsonii |
Synonyms | Comment | Organism |
---|---|---|
acetylacetone dioxygenase | - |
Acinetobacter johnsonii |
Dke1 | - |
Acinetobacter johnsonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.6 | - |
Pentane-2,4-dione | Strep-tagged enzyme | Acinetobacter johnsonii | |
7 | - |
Pentane-2,4-dione | recombinant enzyme | Acinetobacter johnsonii | |
8.5 | - |
Pentane-2,4-dione | native enzyme | Acinetobacter johnsonii |