Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.50 extracted from

  • Grogan, G.
    Emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage (2005), Biochem. J., 388, 721-730.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Acinetobacter johnsonii

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Acinetobacter johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
acetylacetate
-
Acinetobacter johnsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Iron dependent on, metalloenzyme, 1 iron bound per subunit, required for positioning of the substrate and for rendering of the appropriate electronic environment Acinetobacter johnsonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
4 * 16000 Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentane-2,4-dione + O2 Acinetobacter johnsonii
-
acetate + 2-oxopropanal
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
pentane-2,4-dione + O2 = acetate + 2-oxopropanal reaction mechanism Acinetobacter johnsonii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetylacetone-grown cell as sole carbon source Acinetobacter johnsonii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenylbutane-1,3-dione + O2
-
Acinetobacter johnsonii ?
-
?
2-acetylcyclohexanone + O2
-
Acinetobacter johnsonii ?
-
?
3-oxobutanone + O2
-
Acinetobacter johnsonii ?
-
?
additional information the catalytic reaction proceeds via one common rate-limiting step in the clevage of all substrates accepted by the enzyme Acinetobacter johnsonii ?
-
?
pentane-2,4-dione + O2
-
Acinetobacter johnsonii acetate + 2-oxopropanal
-
?
pentane-2,4-dione + O2 i.e. acetylacetone Acinetobacter johnsonii acetate + 2-oxopropanal i.e. acetate and acetone ?

Subunits

Subunits Comment Organism
tetramer 4 * 16000 Acinetobacter johnsonii

Synonyms

Synonyms Comment Organism
diketone-cleaving enzyme
-
Acinetobacter johnsonii
Dke1
-
Acinetobacter johnsonii
pentane-2,4-dione hydrolase
-
Acinetobacter johnsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.5
-
acetylacetate
-
Acinetobacter johnsonii