Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Acinetobacter johnsonii |
Crystallization (Comment) | Organism |
---|---|
- |
Acinetobacter johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0091 | - |
acetylacetate | - |
Acinetobacter johnsonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | dependent on, metalloenzyme, 1 iron bound per subunit, required for positioning of the substrate and for rendering of the appropriate electronic environment | Acinetobacter johnsonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
4 * 16000 | Acinetobacter johnsonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + 2-oxopropanal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pentane-2,4-dione + O2 = acetate + 2-oxopropanal | reaction mechanism | Acinetobacter johnsonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetylacetone-grown cell | as sole carbon source | Acinetobacter johnsonii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenylbutane-1,3-dione + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
2-acetylcyclohexanone + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
3-oxobutanone + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
additional information | the catalytic reaction proceeds via one common rate-limiting step in the clevage of all substrates accepted by the enzyme | Acinetobacter johnsonii | ? | - |
? | |
pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? | |
pentane-2,4-dione + O2 | i.e. acetylacetone | Acinetobacter johnsonii | acetate + 2-oxopropanal | i.e. acetate and acetone | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 16000 | Acinetobacter johnsonii |
Synonyms | Comment | Organism |
---|---|---|
diketone-cleaving enzyme | - |
Acinetobacter johnsonii |
Dke1 | - |
Acinetobacter johnsonii |
pentane-2,4-dione hydrolase | - |
Acinetobacter johnsonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.5 | - |
acetylacetate | - |
Acinetobacter johnsonii |