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Literature summary for 1.13.11.50 extracted from
- Fast determination of operational stability of the soluble acetylacetone-cleaving enzyme Dke1 in an enzyme membrane reactor (2005), Appl. Microbiol. Biotechnol., 1, 1-12.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | biodegradation by the enzyme of the widely used industrial chemical acetylacetone, i.e. 2,4-pentanedione, which has toxic effects, in a membrane bioreactor, determination of operational stability of the enzyme in the reactor at different temperatures, simulations | Acinetobacter johnsonii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21(DE3) | Acinetobacter johnsonii |
General Stability
| General Stability | Organism |
|---|---|
| soluble enzyme is poorly stable | Acinetobacter johnsonii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0034 | - |
Pentane-2,4-dione | pH 7.5, 25°C | Acinetobacter johnsonii |  |
| 0.26 | - |
O2 | pH 7.5, 25°C | Acinetobacter johnsonii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Acinetobacter johnsonii | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + 2-oxopropanal | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter johnsonii | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetylacetone-grown cell | as sole carbon source | Acinetobacter johnsonii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.34 | - |
recombinant enzyme, 35°C | Acinetobacter johnsonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetylacetone-cleaving enzyme | - |
Acinetobacter johnsonii |
| Dke1 | - |
Acinetobacter johnsonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Acinetobacter johnsonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
half-life 308 min in a membrane bioreactor, increase in stability from 5°C to 10°C | Acinetobacter johnsonii |
| 35 | - |
half-life 9 min in a membrane bioreactor | Acinetobacter johnsonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Acinetobacter johnsonii |
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Release 2023.1 (January 2023)
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