Cloned (Comment) | Organism |
---|---|
expression of His-tagged HGO in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation in the absence of O2 due to formation of a white precipitate corresponding to apoprotein | Homo sapiens | |
3-chlorohomogentisate | inactivation in the presence of O2 due to formation of a white precipitate corresponding to apoprotein | Homo sapiens | |
ascorbic acid | 38% inhibition at pH 6.2 | Homo sapiens | |
FeSO4 | 0.25 mM, 42% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
homogentisate | 25°C, pH 8.0 | Homo sapiens | |
0.014 | - |
homogentisate | 37°C, pH 7.0 | Homo sapiens | |
0.015 | - |
homogentisate | 37°C, pH 6.2 | Homo sapiens | |
0.016 | - |
homogentisate | 25°C, pH 7.0 | Homo sapiens | |
0.022 | - |
homogentisate | 25°C, pH 6.2 | Homo sapiens | |
0.042 | - |
homogentisate | 25°C, pH 5.5 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50150 | - |
6 * 50150, mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | Homo sapiens | - |
4-maleylacetoacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged HGO, Ni-affinity, Mono Q | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.3 | - |
recombinant HGO | Homo sapiens |
Storage Stability | Organism |
---|---|
-80, 20 mM Hepps, pH 8.0, 100 mM NaCl, 4 months, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorohomogentisate + O2 | 1% of activity with homogentisate | Homo sapiens | 3-chloro-4-maleylacetoacetate | - |
? | |
3-methylhomogentisate + O2 | 10% of activity with homogentisate | Homo sapiens | 3-methyl-4-maleylacetoacetate | - |
? | |
homogentisate + O2 | - |
Homo sapiens | 4-maleylacetoacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 50150, mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HGO | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
50% loss of activity after 17 s, pH 7.0 | Homo sapiens |
37 | - |
50% loss of activity after 28 s, pH 6.2 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
3-chlorohomogentisate | 25°C, pH 6.2 | Homo sapiens | |
5.3 | - |
3-methylhomogentisate | 25°C, pH 6.2 | Homo sapiens | |
10.1 | - |
homogentisate | 25°C, pH 8.0 | Homo sapiens | |
32.2 | - |
homogentisate | 25°C, pH 7.0 | Homo sapiens | |
41.5 | - |
homogentisate | 25°C, pH 5.5 | Homo sapiens | |
43.4 | - |
homogentisate | 37°C, pH 7.0 | Homo sapiens | |
56 | - |
homogentisate | 25°C, pH 6.2 | Homo sapiens | |
64.3 | - |
homogentisate | 37°C, pH 6.2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
Good buffer | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | approx. 15% of maximal activity at pH 5.0 and pH 8.0, respectively | Homo sapiens |