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Literature summary for 1.13.11.5 extracted from

  • Veldhuizen, E.J.; Vaillancourt, F.H.; Whiting, C.J.; Hsiao, M.M.; Gingras, G.; Xiao, Y.; Tanguay, R.M.; Boukouvalas, J.; Eltis, L.D.
    Steady-state kinetics and inhibition of anaerobically purified human homogentisate 1,2-dioxygenase (2005), Biochem. J., 386, 305-314.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged HGO in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inactivation in the absence of O2 due to formation of a white precipitate corresponding to apoprotein Homo sapiens
3-chlorohomogentisate inactivation in the presence of O2 due to formation of a white precipitate corresponding to apoprotein Homo sapiens
ascorbic acid 38% inhibition at pH 6.2 Homo sapiens
FeSO4 0.25 mM, 42% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
homogentisate 25°C, pH 8.0 Homo sapiens
0.014
-
homogentisate 37°C, pH 7.0 Homo sapiens
0.015
-
homogentisate 37°C, pH 6.2 Homo sapiens
0.016
-
homogentisate 25°C, pH 7.0 Homo sapiens
0.022
-
homogentisate 25°C, pH 6.2 Homo sapiens
0.042
-
homogentisate 25°C, pH 5.5 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50150
-
6 * 50150, mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
homogentisate + O2 Homo sapiens
-
4-maleylacetoacetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HGO, Ni-affinity, Mono Q Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.3
-
recombinant HGO Homo sapiens

Storage Stability

Storage Stability Organism
-80, 20 mM Hepps, pH 8.0, 100 mM NaCl, 4 months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorohomogentisate + O2 1% of activity with homogentisate Homo sapiens 3-chloro-4-maleylacetoacetate
-
?
3-methylhomogentisate + O2 10% of activity with homogentisate Homo sapiens 3-methyl-4-maleylacetoacetate
-
?
homogentisate + O2
-
Homo sapiens 4-maleylacetoacetate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 50150, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
HGO
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
50% loss of activity after 17 s, pH 7.0 Homo sapiens
37
-
50% loss of activity after 28 s, pH 6.2 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
3-chlorohomogentisate 25°C, pH 6.2 Homo sapiens
5.3
-
3-methylhomogentisate 25°C, pH 6.2 Homo sapiens
10.1
-
homogentisate 25°C, pH 8.0 Homo sapiens
32.2
-
homogentisate 25°C, pH 7.0 Homo sapiens
41.5
-
homogentisate 25°C, pH 5.5 Homo sapiens
43.4
-
homogentisate 37°C, pH 7.0 Homo sapiens
56
-
homogentisate 25°C, pH 6.2 Homo sapiens
64.3
-
homogentisate 37°C, pH 6.2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
Good buffer Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8 approx. 15% of maximal activity at pH 5.0 and pH 8.0, respectively Homo sapiens