Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli is fully active in absence of ascorbate | Aspergillus nidulans |
Cloned (Comment) | Organism |
---|---|
hmgA gene encoding homogentisate dioxygenase, a 448 residue polypeptide, is cloned, sequenced and characterized, gene is expressed in Escherichia coli as a fusion to glutathione S-transferase | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain biA1, methG1, argB2 with homogentisate dioxygenase gene disruption has no homogentisate dioxygenase activity and accumulates homogentisate | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 70% reduction of enzyme activity in crude extract in absence of Fe2+ and absolutely requirement for Fe2+ of the fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50168 | - |
x * 50168, calculated from the amino acid sequence | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | Aspergillus nidulans | catalyzes an essential step in phenylalanine catabolism | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | Aspergillus nidulans BiA1 | catalyzes an essential step in phenylalanine catabolism | 4-maleylacetoacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q00667 | - |
- |
Aspergillus nidulans | Q00667 | filamentous ascomycete | - |
Aspergillus nidulans BiA1 | Q00667 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of a fusion protein of homogentisate dioxygenase and glutathione S-transferase, expressed in Escherichia coli | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | - |
Aspergillus nidulans | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | catalyzes an essential step in phenylalanine catabolism | Aspergillus nidulans | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | - |
Aspergillus nidulans BiA1 | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | catalyzes an essential step in phenylalanine catabolism | Aspergillus nidulans BiA1 | 4-maleylacetoacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50168, calculated from the amino acid sequence | Aspergillus nidulans |