Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of methionine can partly rescue inactivation during the reaction | Nitrospira defluvii |
Cloned (Comment) | Organism |
---|---|
expresssion in Escherichia coli | Nitrospira defluvii |
Protein Variants | Comment | Organism |
---|---|---|
R173A | mutation increases the extent of irreversible inactivation. In the presence of the hypochlorite traps methionine, monochlorodimedone, and 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid, the extent of chlorite degradation and release of molecular oxygen is significantly increased, whereas heme bleaching and oxidative modifications of the protein are suppressed | Nitrospira defluvii |
R173K | mutation increases the extent of irreversible inactivation. In the presence of the hypochlorite traps methionine, monochlorodimedone, and 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid, the extent of chlorite degradation and release of molecular oxygen is significantly increased, whereas heme bleaching and oxidative modifications of the protein are suppressed | Nitrospira defluvii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
Chlorite | wild-type, pH 7.0, 30°C | Nitrospira defluvii | |
0.09 | - |
Chlorite | mutant R173A, pH 7.0, 30°C | Nitrospira defluvii | |
0.898 | - |
Chlorite | mutant R173K, pH 7.0, 30°C | Nitrospira defluvii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | Nitrospira defluvii | - |
chloride + O2 | - |
? | |
additional information | Nitrospira defluvii | upon reduction of chlorite, hypochlorite is formed and kept in the reaction sphere for recombination with the oxoiron(IV) group of Compound I. Approximately one molecule of HOCl per 100 full cycles escapes and reacts with both the prosthetic group and the protein moiety, leading to irreversible inactivation of Cld is observed which is more pronounced with an increase in pH. HOCl traps like methionine can rescue the enzyme from inactivation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrospira defluvii | B3U4H7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | - |
Nitrospira defluvii | chloride + O2 | - |
? | |
additional information | upon reduction of chlorite, hypochlorite is formed and kept in the reaction sphere for recombination with the oxoiron(IV) group of Compound I. Approximately one molecule of HOCl per 100 full cycles escapes and reacts with both the prosthetic group and the protein moiety, leading to irreversible inactivation of Cld is observed which is more pronounced with an increase in pH. HOCl traps like methionine can rescue the enzyme from inactivation | Nitrospira defluvii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CLD | - |
Nitrospira defluvii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Chlorite | mutant R173A, pH 7.0, 30°C | Nitrospira defluvii | |
14 | - |
Chlorite | mutant R173K, pH 7.0, 30°C | Nitrospira defluvii | |
43 | - |
Chlorite | wild-type, pH 7.0, 30°C | Nitrospira defluvii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
KM value is independent of pH | Nitrospira defluvii |
4.5 | - |
mutants R173A and R173K | Nitrospira defluvii |
5.5 | - |
wild-type | Nitrospira defluvii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
Chlorite | mutant R173K, pH 7.0, 30°C | Nitrospira defluvii | |
31 | - |
Chlorite | mutant R173A, pH 7.0, 30°C | Nitrospira defluvii | |
620 | - |
Chlorite | wild-type, pH 7.0, 30°C | Nitrospira defluvii |