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Literature summary for 1.13.11.49 extracted from
- Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases (2012), Biochim. Biophys. Acta, 1824, 1031-1038.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the enzyme from Nitrospira defluvii is an interesting candidate for bioremediation of chlorite | Nitrospira defluvii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His-tagged enzyme in Escherichia coli strain Tuner (DE3) | Nitrobacter winogradskyi |
| recombinant expression of the enzyme without the N-terminal signal, but with N-terminal TEV-cleavable Strep-II tag in Escherichia coli strain Tuner (DE3) | Nitrospira defluvii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, overview | Nitrospira defluvii | |
| additional information | - |
additional information | steady-state kinetics, overview | Nitrobacter winogradskyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorite | Nitrospira defluvii | - |
chloride + O2 | - |
? |  |
| chlorite | Nitrobacter winogradskyi | - |
chloride + O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nitrobacter winogradskyi | Q3SPU6 | - |
- |
| Nitrospira defluvii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography | Nitrobacter winogradskyi |
| recombinant Strep-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography, the Strep-II-tag is fully cleaved off using TEV-protease, followed by gel filtration | Nitrospira defluvii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorite | - |
Nitrospira defluvii | chloride + O2 | - |
? | |
| chlorite | - |
Nitrobacter winogradskyi | chloride + O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview | Nitrobacter winogradskyi |
| pentamer | conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview | Nitrospira defluvii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chlorite dismutase | - |
Nitrospira defluvii |
| chlorite dismutase | - |
Nitrobacter winogradskyi |
| CLD | - |
Nitrospira defluvii |
| CLD | - |
Nitrobacter winogradskyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Nitrospira defluvii |
| 25 | - |
assay at | Nitrobacter winogradskyi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | activity range | Nitrobacter winogradskyi |
| 30 | 90 | activity range | Nitrospira defluvii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview | Nitrobacter winogradskyi |
| additional information | - |
conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview | Nitrospira defluvii |
| 40 | - |
purified recombinant enzyme, stable up to | Nitrospira defluvii |
| 40 | - |
purified recombinant enzyme, stable up to | Nitrobacter winogradskyi |
| 50 | - |
purified recombinant enzyme, 80% activity remaining | Nitrospira defluvii |
| 50 | - |
purified recombinant enzyme, 8% activity remaining | Nitrobacter winogradskyi |
| 55 | - |
purified recombinant enzyme, inactivation | Nitrobacter winogradskyi |
| 70 | - |
purified recombinant enzyme, 36% activity remaining | Nitrospira defluvii |
| 90 | - |
purified recombinant enzyme, inactivation | Nitrospira defluvii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Nitrospira defluvii |
| 6 | - |
- |
Nitrobacter winogradskyi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the pentameric enzyme is very stable between pH 3 and 10 with Tm=92°C at pH 7.0 | Nitrospira defluvii |
| 7 | - |
purified recombinant enzyme, stable | Nitrospira defluvii |
| 7 | - |
purified recombinant enzyme, stable | Nitrobacter winogradskyi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview | Nitrobacter winogradskyi |
| additional information | conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview | Nitrospira defluvii |
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Release 2023.1 (January 2023)
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