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Literature summary for 1.13.11.47 extracted from

  • Bauer, I.; Max, N.; Fetzner, S.; Lingens, F.
    2,4-Dioxygenases catalyzing N-heterocyclic-ring cleavage and formation of carbon monoxide. Purification and some properties of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter sp. Rii61a and comparison with 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from Pseudomonas putida 33/1 (1996), Eur. J. Biochem., 240, 576-583.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
ascorbate
-
 Arthrobacter sp.
Ca2+ weak Arthrobacter sp.
Co2+ weak Arthrobacter sp.
Cu2+ strong Arthrobacter sp.
Cu2+
-
 Pseudomonas putida
ethylxanthate
-
 Arthrobacter sp.
Fe2+ strong Arthrobacter sp.
H2O2
-
 Arthrobacter sp.
H2O2
-
 Pseudomonas putida
iodoacetamide
-
 Arthrobacter sp.
Mg2+ weak Arthrobacter sp.
Mn2+ weak Arthrobacter sp.
NEM
-
 Arthrobacter sp.
Ni2+ strong Arthrobacter sp.
p-hydroxymercuribenzoate
-
 Arthrobacter sp.
Sodium azide
-
 Arthrobacter sp.
Sodium dithionite
-
 Arthrobacter sp.
Zn2+ strong Arthrobacter sp.
Zn2+
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 1H-3-Hydroxy-4-oxoquinoline
-
 Pseudomonas putida
0.03
-
 1H-3-Hydroxy-4-oxoquinaldine
-
 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains 0.048 mol of Cu per mol of enzyme Pseudomonas putida
additional information addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity Pseudomonas putida
additional information addition of metal ions in the absence and in the presence of the reductant ascorbate does not increase activity Arthrobacter sp.
Nickel contains 0.038 mol of Ni per mol of enzyme Pseudomonas putida
Nickel contains 0.02 mol of Ni per mol of enzyme Arthrobacter sp.
Zinc contains 0.045 mol of zinc per mol of enzyme Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Pseudomonas putida
32000
-
 gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 strain R³61a
-
Arthrobacter sp. Ru61a
-
 strain R³61a
-
Pseudomonas putida
-
-
-
Pseudomonas putida 33/1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida
-
 Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:quinaldine-grown cell
-
 Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.1
-
-
 Pseudomonas putida
70.7
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1H-3-Hydroxy-4-oxoquinaldine + O2
-
 Arthrobacter sp. N-Acetylanthranilate + CO
-
 ?
1H-3-Hydroxy-4-oxoquinaldine + O2
-
 Arthrobacter sp. Ru61a N-Acetylanthranilate + CO
-
 ?
1H-3-Hydroxy-4-oxoquinoline + O2
-
 Pseudomonas putida N-Formylanthranilate + CO
-
 ?
1H-3-Hydroxy-4-oxoquinoline + O2 at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine Arthrobacter sp. N-Formylanthranilate + CO
-
 ?
1H-3-Hydroxy-4-oxoquinoline + O2
-
 Pseudomonas putida 33/1 N-Formylanthranilate + CO
-
 ?
1H-3-Hydroxy-4-oxoquinoline + O2 at 20% of the activity with 1H-3-Hydroxy-4-oxoquinaldine Arthrobacter sp. Ru61a N-Formylanthranilate + CO
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Pseudomonas putida
monomer 1 * 32000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas putida
30
-
-
 Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 labile above Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida
8
-
-
 Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
additional information no spectral evidence for the presence of a chromophoric cofactor Pseudomonas putida
additional information no spectral evidence for the presence of a chromophoric cofactor Arthrobacter sp.