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Literature summary for 1.13.11.45 extracted from

  • Cristea, M.; Oliw, E.H.
    A G316A mutation of manganese lipoxygenase augments hydroperoxide isomerase activity: mechanism of biosynthesis of epoxyalcohols (2006), J. Biol. Chem., 281, 17612-17623.
    View publication on PubMed
Search for more literature for 1.13.11.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Gaeumannomyces graminis

Protein Variants

Protein Variants Comment Organism
G316A catalytically active, 7-8% of hydroperoxide isomerase activity Gaeumannomyces graminis
G316T catalytically inactive Gaeumannomyces graminis
G316V catalytically inactive Gaeumannomyces graminis

Inhibitors

Inhibitors Comment Organism Structure
eicosatetraynoic acid 0.1 mM, 75% inhibition Gaeumannomyces graminis
N-(3-phenoxycinnamyl) acetohydroxamic acid 0.3 mM, complete inhibition Gaeumannomyces graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 (13R)-hydroperoxylinolenic acid mutant enzyme G316A Gaeumannomyces graminis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis Q8X151 variant avenae
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13R)-hydroperoxylinolenic acid + O2 only mutant enzyme G316A Gaeumannomyces graminis 13-ketolinolenic acid + epoxyalcohols erythro- and threo-11-hydroxy-(12R,13R)-epoxy-(9Z,15Z)-octadecadienoic acids and one of the corresponding cis-epoxides as major products ?
linoleic acid + O2
-
 Gaeumannomyces graminis (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
 ?

Synonyms

Synonyms Comment Organism
Mn-LO
-
 Gaeumannomyces graminis