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Literature summary for 1.13.11.43 extracted from

  • Kamoda, S.; Habu, N.; Samejima, M.; Yoshimoto, T.
    Purification and some properties of lignostilbene-alpha,beta-dioxygenase responsible for the Calpha-Cbeta cleavage of a diarylpropane type lignin model compound from Pseudomonas sp. TMY1009 (1989), Agric. Biol. Chem., 53, 2757-2761.
No PubMed abstract available
Search for more literature for 1.13.11.43

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene isozyme I Sphingomonas paucimobilis
0.11
-
 O2 isozyme I Sphingomonas paucimobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Sphingomonas paucimobilis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 1 g-atom per mol enzyme Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 alpha2, 2 * 52000, SDS-PAGE, isozyme I Sphingomonas paucimobilis
94000
-
 gel filtration, isozyme I, II, III Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
stilbene + O2 Sphingomonas paucimobilis reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin ?
-
 ?
stilbene + O2 Sphingomonas paucimobilis TMY 1009 reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
 formerly Pseudomonas sp.
-
Sphingomonas paucimobilis
-
 four isoenzymes
-
Sphingomonas paucimobilis TMY 1009
-
 four isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
major isozyme I, 4 isozymes by ion-exchange chromatography Sphingomonas paucimobilis

Storage Stability

Storage Stability Organism
4°C, one week, 70% activity, isozyme I Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2 stilbene-type intermediate of diarylpropane-type lignin model compound Sphingomonas paucimobilis vanillin
-
 ?
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2 stilbene-type intermediate of diarylpropane-type lignin model compound Sphingomonas paucimobilis TMY 1009 vanillin
-
 ?
additional information catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol Sphingomonas paucimobilis ?
-
 ?
additional information catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol Sphingomonas paucimobilis TMY 1009 ?
-
 ?
stilbene + O2 reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin Sphingomonas paucimobilis ?
-
 ?
stilbene + O2 reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin Sphingomonas paucimobilis TMY 1009 ?
-
 ?
trans-4-hydroxystilbene + O2 oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation Sphingomonas paucimobilis ?
-
 ?
trans-4-hydroxystilbene + O2 oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation Sphingomonas paucimobilis TMY 1009 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer alpha2, 2 * 52000, SDS-PAGE, isozyme I Sphingomonas paucimobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 after 3 h, 100% activity retained, isozyme 1 Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 isozyme I Sphingomonas paucimobilis