Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plexaura homomalla |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion in 6.25% polyethylene glycol 8000, 100 mM imidazole acetate (pH 8.0), 100 mM CaCl2, and 5% sucrose at 22°C | Plexaura homomalla |
Protein Variants | Comment | Organism |
---|---|---|
D39A | 118% activity compared to the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding | Plexaura homomalla |
D39A/E47A | 106% activity compared to the wild type enzyme, a double mutant with calcium-binding residues from two of the three sites mutated exhibits no fluorescence resonance energy transfer signal | Plexaura homomalla |
E47A | 65% of the activity of the wild type enzyme, mutant with diminished fluorescence resonance energy transfer properties, consistent with a role for calcium in membrane binding | Plexaura homomalla |
W41A | 140% activity compared to the wild type enzyme, exhibits only less than 2% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal | Plexaura homomalla |
W77A | 44% of the activity of the wild type enzyme, exhibits only 4% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal | Plexaura homomalla |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+-regulated enzyme activity | Plexaura homomalla |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plexaura homomalla | - |
Caribbean sea whip coral | - |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid-agarose chromatography, DE52 column chromatography, and Mono Q column chromatography | Plexaura homomalla |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + O2 | - |
Plexaura homomalla | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
8R-lipoxygenase | - |
Plexaura homomalla |
8R-LOX | - |
Plexaura homomalla |