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Literature summary for 1.13.11.4 extracted from
- The salicylate 1,2-dioxygenase as a model for a conventional gentisate 1,2-dioxygenase: crystal structures of the G106A mutant and its adducts with gentisate and salicylate (2013), FEBS J., 280, 1643-1652.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes | Pseudaminobacter salicylatoxidans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified G106A enzyme mutant free and complexes with gentisate and salicylate, crystallization under anaerobic conditions, sitting drop vapor diffusion method, mixing of 0.001 ml of protein solution with 0.0008 ml of 8% poly(ethylene glycol)10000 and 0.1 M Tris/HCl pH 8.0, and 0.0002 ml of 0.1 M calcium chloride, crystal quality is improved using the seeding technique, X-ray diffraction structure determination and analysis at 2.0-2.58 A resolution | Pseudaminobacter salicylatoxidans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F159Y | site-directed mutagenesis | Pseudaminobacter salicylatoxidans |
| G106A | site-directed mutagenesis, in contrast to the wild-type enzyme, mutant G106A oxidizes only gentisate, while 1-hydroxy-2-naphthoate and salicylate are not converted. The mutant shows slightly decreased activity with salicylate, but shows a higher affinity to this substratecompared to the wild-type. Salicylate is coordinated in the G106A variant with the catalytically active Fe(II) ion in an unusual and unproductive manner because of the inability of salicylate to displace a hydrogen bond that is formed between Trp104 and Asp174 in the G106A variant | Pseudaminobacter salicylatoxidans |
| G111A | site-directed mutagenesis | Pseudaminobacter salicylatoxidans |
| M103L | site-directed mutagenesis | Pseudaminobacter salicylatoxidans |
| R113G | site-directed mutagenesis | Pseudaminobacter salicylatoxidans |
| S147R | site-directed mutagenesis | Pseudaminobacter salicylatoxidans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
salicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans |  |
| 0.047 | - |
5-Fluorosalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.14 | - |
1-hydroxynaphthalene-2-carboxylic acid | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.167 | - |
gentisate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.216 | - |
5-Aminosalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.788 | - |
5-Methylsalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | catalytically active Fe(II) ion | Pseudaminobacter salicylatoxidans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gentisate + O2 | Pseudaminobacter salicylatoxidans | - |
maleylpyruvate | - |
? | |
| gentisate + O2 | Pseudaminobacter salicylatoxidans BN12 | - |
maleylpyruvate | - |
? | |
| additional information | Pseudaminobacter salicylatoxidans | the salicylate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans strain BN12 is a versatile gentisate 1,2-dioxygenase that converts both gentisate (2,5-dihydroxybenzoate) and various monohydroxylated substrates | ? | - |
? | |
| additional information | Pseudaminobacter salicylatoxidans BN12 | the salicylate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans strain BN12 is a versatile gentisate 1,2-dioxygenase that converts both gentisate (2,5-dihydroxybenzoate) and various monohydroxylated substrates | ? | - |
? | |
| salicylate + O2 | Pseudaminobacter salicylatoxidans | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudaminobacter salicylatoxidans | - |
- |
- |
| Pseudaminobacter salicylatoxidans BN12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mutant G106A | Pseudaminobacter salicylatoxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-2-naphthoate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
| 1-hydroxy-2-naphthoate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| 5-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
| 5-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| 5-fluorosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
| 5-fluorosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| 5-methylsalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
| gentisate + O2 | - |
Pseudaminobacter salicylatoxidans | maleylpyruvate | - |
? | |
| gentisate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | maleylpyruvate | - |
? | |
| additional information | the salicylate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans strain BN12 is a versatile gentisate 1,2-dioxygenase that converts both gentisate (2,5-dihydroxybenzoate) and various monohydroxylated substrates | Pseudaminobacter salicylatoxidans | ? | - |
? | |
| additional information | the salicylate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans strain BN12 is a versatile gentisate 1,2-dioxygenase that converts both gentisate (2,5-dihydroxybenzoate) and various monohydroxylated substrates | Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| salicylate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDO | - |
Pseudaminobacter salicylatoxidans |
| salicylate 1,2-dioxygenase | - |
Pseudaminobacter salicylatoxidans |
| SDO | - |
Pseudaminobacter salicylatoxidans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
salicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.1 | - |
5-Methylsalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.4 | - |
5-Fluorosalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.8 | - |
5-Aminosalicylate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 24 | - |
1-hydroxynaphthalene-2-carboxylic acid | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 133 | - |
gentisate | wild-type enzyme, pH and temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | binding modes for salicylate and gentisate | Pseudaminobacter salicylatoxidans |
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