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Literature summary for 1.13.11.4 extracted from
- Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi (2007), Biotechnol. Lett., 29, 1529-1535.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Ruegeria pomeroyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 2 mM, complete inhibition | Ruegeria pomeroyi |  |
| EDTA | 100 mM, complete inhibition | Ruegeria pomeroyi | |
| Fe3+ | 2 mM | Ruegeria pomeroyi | |
| Hg2+ | 2 mM, complete inhibition | Ruegeria pomeroyi | |
| Mn2+ | 2 mM | Ruegeria pomeroyi | |
| Zn2+ | 2 mM | Ruegeria pomeroyi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
2,5-Dihydroxybenzoate | - |
Ruegeria pomeroyi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Ruegeria pomeroyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
3 * 45000, SDS-PAGE | Ruegeria pomeroyi |
| 132000 | - |
gel filtration | Ruegeria pomeroyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruegeria pomeroyi | Q5LLB1 | DSS-3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Ruegeria pomeroyi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 week, purified enzyme loses 51% of its initial activity | Ruegeria pomeroyi |
| -70°C, 1 week, purified enzyme loses 30% of its initial activity | Ruegeria pomeroyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dihydroxybenzoate + O2 | - |
Ruegeria pomeroyi | maleylpyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 45000, SDS-PAGE | Ruegeria pomeroyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Ruegeria pomeroyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ruegeria pomeroyi |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ruegeria pomeroyi | isoelectric focusing | 4.8 | 4.6 |
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