Any feedback?
Literature summary for 1.13.11.4 extracted from
- Structural and biochemical characterization of gentisate 1,2-dioxygenase from Escherichia coli O157:H7 (2006), Mol. Microbiol., 61, 1469-1484.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method using 0.1 M BisTris pH 6.5 and 20% polyethylene glycol 5000 monomethylether | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli |  |
| EDTA | complete inhibition at pH 8.0 | Escherichia coli | |
| Li2SO4 | 5 mM 5-10% inhibition at pH 8.0 | Escherichia coli | |
| MgSO4 | 5 mM, 5-10% inhibition at pH 8.0 | Escherichia coli | |
| Mn2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli | |
| additional information | no inhibition by 5 mM Ca2+ and K+ | Escherichia coli | |
| Na2SO4 | 5 mM, 5-10% inhibition at pH 8.0 | Escherichia coli | |
| Zn2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
2,5-Dihydroxybenzoate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | contains 1 mol iron per subunit, in the presence of 2 mM Fe2+ activity is increased by 115% | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38500 | - |
4 * 38500, gel filtration | Escherichia coli |
| 38900 | - |
SDS-PAGE | Escherichia coli |
| 154000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q8X655 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-nitriloacetic acid resin column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dihydroxybenzoate + O2 | - |
Escherichia coli | maleylpyruvate | - |
? | |
| additional information | methyl 2,5-dihydroxybenzoate, 1,4-benzoquinone, 2,5-dihydroxybenzaldehyde, 4,5-dihydroxy-1,3-benzene-disulphonic acid and 2,3,5,6-tetrachloro-1,4-benzenediol give no substrate activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38500, gel filtration | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| eGDO | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
