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Literature summary for 1.13.11.4 extracted from

  • Luo, S.; Liu, D.Q.; Liu, H.; Zhou, N.Y.
    Site-directed mutagenesis of gentisate 1,2-dioxygenases from Klebsiella pneumoniae M5a1 and Ralstonia sp. strain U2 (2006), Microbiol. Res., 161, 138-144.
    View publication on PubMed
Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells Klebsiella pneumoniae
expressed in Escherichia coli Rosetta cells Ralstonia sp.

Protein Variants

Protein Variants Comment Organism
H108D complete loss of activity Klebsiella pneumoniae
H108D complete loss of activity Ralstonia sp.
H110D complete loss of activity Klebsiella pneumoniae
H110D complete loss of activity Ralstonia sp.
H118D complete loss of activity Klebsiella pneumoniae
H118D complete loss of activity Ralstonia sp.
H120D complete loss of activity Klebsiella pneumoniae
H120D complete loss of activity Ralstonia sp.
H149D complete loss of activity Klebsiella pneumoniae
H149D complete loss of activity Ralstonia sp.
H151D complete loss of activity Klebsiella pneumoniae
H151D complete loss of activity Ralstonia sp.
H159D complete loss of activity Klebsiella pneumoniae
H159D complete loss of activity Ralstonia sp.
H161D complete loss of activity Klebsiella pneumoniae
H161D complete loss of activity Ralstonia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Klebsiella pneumoniae
35000
-
 SDS-PAGE Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae M5a1
-
-
-
Ralstonia sp.
-
-
-
Ralstonia sp. U2
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
 Klebsiella pneumoniae maleylpyruvate
-
 ir
2,5-dihydroxybenzoate + O2
-
 Ralstonia sp. maleylpyruvate
-
 ir
2,5-dihydroxybenzoate + O2
-
 Ralstonia sp. U2 maleylpyruvate
-
 ir
2,5-dihydroxybenzoate + O2
-
 Klebsiella pneumoniae M5a1 maleylpyruvate
-
 ir

Synonyms

Synonyms Comment Organism
GDO
-
 Klebsiella pneumoniae
GDO
-
 Ralstonia sp.