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Literature summary for 1.13.11.4 extracted from

  • Crawford, R.I.; Hutton, S.W.; Chapman, P.J.
    Purification and properties of gentisate 1,2-dioxygenase from Moraxella osloensis (1975), J. Bacteriol., 121, 794-799.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.4

Activating Compound

Activating Compound Comment Organism Structure
Fe2+
-
 Moraxella osloensis

General Stability

General Stability Organism
Fe2+ stabilizes Moraxella osloensis

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 0.05 mM, over 90% inhibition Moraxella osloensis
o-phenanthroline 0.05 mM, over 90% inhibition Moraxella osloensis
Tiron 4,5-dihydroxy-m-benzene disulfonic acid, 0.17 mM, 23% inhibition Moraxella osloensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
 gentisate
-
 Moraxella osloensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Moraxella osloensis
154000
-
 gel filtration Moraxella osloensis

Organism

Organism UniProt Comment Textmining
Moraxella osloensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Moraxella osloensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
 Moraxella osloensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
 Moraxella osloensis maleylpyruvate
-
 ?
3-bromogentisate + O2
-
 Moraxella osloensis 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
3-chlorogentisate + O2
-
 Moraxella osloensis 3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
3-ethylgentisate + O2
-
 Moraxella osloensis 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
3-fluorogentisate + O2
-
 Moraxella osloensis 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
3-methylgentisate + O2
-
 Moraxella osloensis 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
 ?
4-chlorogentisate + O2
-
 Moraxella osloensis 2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
4-fluorogentisate + O2
-
 Moraxella osloensis 2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
 ?
4-methylgentisate + O2
-
 Moraxella osloensis 4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
-
 ?
additional information broad substrate specificity Moraxella osloensis ?
-
 ?
additional information no activity with: 6-methylgentisate, 3,4-dimethylgentisate Moraxella osloensis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Moraxella osloensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Moraxella osloensis