Cloned (Comment) | Organism |
---|---|
genes BphC1 and BphC2 cloned and expressed in Escherichia coli JM108 and JM109 | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-chlorocatechol | competitive inhibition | Sphingomonas sp. | |
o-phenanthroline | - |
Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
2,3-Dihydroxybiphenyl | in presence of 3-chlorocatechol | Sphingomonas sp. | |
0.007 | - |
3,5-dichlorocatechol | Glu79His mutant | Sphingomonas sp. | |
0.025 | - |
3-chlorocatechol | Glu79His mutant | Sphingomonas sp. | |
0.03 | - |
3,5-dichlorocatechol | - |
Sphingomonas sp. | |
0.032 | - |
2,3-Dihydroxybiphenyl | Glu79His mutant | Sphingomonas sp. | |
0.097 | - |
3-methylcatechol | Glu79His mutant | Sphingomonas sp. | |
0.1 | - |
3-chlorocatechol | - |
Sphingomonas sp. | |
0.18 | - |
3-methylcatechol | - |
Sphingomonas sp. | |
0.67 | - |
catechol | Glu79His mutant | Sphingomonas sp. | |
0.67 | - |
catechol | mutant enzyme E79H, at pH 7.5, temperature not specified in the publication | Sphingomonas sp. | |
0.74 | - |
2,3-Dihydroxybiphenyl | - |
Sphingomonas sp. | |
1.13 | - |
catechol | wild type enzyme, at pH 7.5, temperature not specified in the publication | Sphingomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cofactor in the catalytic center | Sphingomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | Sphingomonas sp. | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Rhodococcus globerulus | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Sphingomonas sp. BN6 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | Rhodococcus globerulus P6 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus globerulus | - |
- |
- |
Rhodococcus globerulus P6 | - |
- |
- |
Sphingomonas sp. | - |
- |
- |
Sphingomonas sp. BN6 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
BphC1 | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dichlorocatechol + O2 | - |
Sphingomonas sp. | 3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3,5-dichlorocatechol + O2 | - |
Sphingomonas sp. BN6 | 3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
3-chlorocatechol + O2 | - |
Sphingomonas sp. | 3-chloro-2-hydroxymuconic semialdehyde + H2O | - |
? | |
3-chlorocatechol + O2 | - |
Sphingomonas sp. BN6 | 3-chloro-2-hydroxymuconic semialdehyde + H2O | - |
? | |
3-methylcatechol + O2 | - |
Sphingomonas sp. | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Sphingomonas sp. BN6 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | - |
Sphingomonas sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Rhodococcus globerulus | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Sphingomonas sp. BN6 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
biphenyl-2,3-diol + O2 | - |
Rhodococcus globerulus P6 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
catechol + O2 | - |
Sphingomonas sp. | cis,cis-muconate | - |
r | |
catechol + O2 | - |
Sphingomonas sp. BN6 | cis,cis-muconate | - |
r | |
catechol + O2 | - |
Sphingomonas sp. | 2-hydroxymuconate semialdehyde | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BphC1-BN6 | - |
Sphingomonas sp. |
BphC2-BN6 | - |
Sphingomonas sp. |