Literature summary for 1.13.11.39 extracted from

Riegert, U.; Heiss, G.; Kuhm, A.E.; Mueller, C.; Contzen, M.; Knackmuss, H.J.; Stolz, A.
Catalytic properties of the 3-chlorocatechol-oxidizing 2,3-dihydroxybiphenyl 1,2-dioxygenase from Sphingomonas sp. strain BN6 (1999), J. Bacteriol., 181, 4812-4817.

Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment)	Organism
genes BphC1 and BphC2 cloned and expressed in Escherichia coli JM108 and JM109	Sphingomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
3-chlorocatechol	competitive inhibition	Sphingomonas sp.
o-phenanthroline	-	Sphingomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	2,3-Dihydroxybiphenyl	in presence of 3-chlorocatechol	Sphingomonas sp.
0.007	-	3,5-dichlorocatechol	Glu79His mutant	Sphingomonas sp.
0.025	-	3-chlorocatechol	Glu79His mutant	Sphingomonas sp.
0.03	-	3,5-dichlorocatechol	-	Sphingomonas sp.
0.032	-	2,3-Dihydroxybiphenyl	Glu79His mutant	Sphingomonas sp.
0.097	-	3-methylcatechol	Glu79His mutant	Sphingomonas sp.
0.1	-	3-chlorocatechol	-	Sphingomonas sp.
0.18	-	3-methylcatechol	-	Sphingomonas sp.
0.67	-	catechol	Glu79His mutant	Sphingomonas sp.
0.67	-	catechol	mutant enzyme E79H, at pH 7.5, temperature not specified in the publication	Sphingomonas sp.
0.74	-	2,3-Dihydroxybiphenyl	-	Sphingomonas sp.
1.13	-	catechol	wild type enzyme, at pH 7.5, temperature not specified in the publication	Sphingomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	cofactor in the catalytic center	Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
biphenyl-2,3-diol + O2	Sphingomonas sp.	-	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	Rhodococcus globerulus	-	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	Sphingomonas sp. BN6	-	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	Rhodococcus globerulus P6	-	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus globerulus	-	-	-
Rhodococcus globerulus P6	-	-	-
Sphingomonas sp.	-	-	-
Sphingomonas sp. BN6	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1	-	BphC1	Sphingomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,5-dichlorocatechol + O2	-	Sphingomonas sp.	3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate	-	?
3,5-dichlorocatechol + O2	-	Sphingomonas sp. BN6	3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate	-	?
3-chlorocatechol + O2	-	Sphingomonas sp.	3-chloro-2-hydroxymuconic semialdehyde + H2O	-	?
3-chlorocatechol + O2	-	Sphingomonas sp. BN6	3-chloro-2-hydroxymuconic semialdehyde + H2O	-	?
3-methylcatechol + O2	-	Sphingomonas sp.	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
3-methylcatechol + O2	-	Sphingomonas sp. BN6	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
biphenyl-2,3-diol + O2	-	Sphingomonas sp.	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	-	Rhodococcus globerulus	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	-	Sphingomonas sp. BN6	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
biphenyl-2,3-diol + O2	-	Rhodococcus globerulus P6	2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O	-	?
catechol + O2	-	Sphingomonas sp.	cis,cis-muconate	-	r
catechol + O2	-	Sphingomonas sp. BN6	cis,cis-muconate	-	r
catechol + O2	-	Sphingomonas sp.	2-hydroxymuconate semialdehyde	-	r

Synonyms

Synonyms	Comment	Organism
BphC1-BN6	-	Sphingomonas sp.
BphC2-BN6	-	Sphingomonas sp.

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Release 2023.1 (January 2023)