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Literature summary for 1.13.11.39 extracted from

  • Khan, A.A.; Nawaz, M.S.; Cerniglia, C.E.
    Rapid purification of an active recombinant His-tagged 2,3-dihydroxybiphenyl 1,2-dioxygenase from Pseudomonas putida OU83 (1997), FEMS Microbiol. Lett., 152, 23-29.
    View publication on PubMed
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas oleovorans
bphC gene cloned Pseudomonas aeruginosa
nucleotide sequence of gene bphC cloned into pQE1 Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 5 mM 30 min Pseudomonas putida
8-hydroxyquinoline 5 mM 30 min Pseudomonas putida
CdCl2
-
 Pseudomonas putida
CuSO4
-
 Pseudomonas putida
diethyldithiocarbamic acid 5 mM 30 min Pseudomonas putida
Fe3+
-
 Pseudomonas putida
FeCl3
-
 Pseudomonas putida
HgCl2
-
 Pseudomonas putida
iodoacetic acid 5 mM 30 min Pseudomonas putida
N-ethylmaleimide 5 mM 30 min Pseudomonas putida
NiSO4
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
 2,3-Dihydroxybiphenyl strain OU83 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Comamonas testosteroni
Fe2+ significantly enhances activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Pseudomonas putida
270000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Pseudomonas aeruginosa
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Cupriavidus necator
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Comamonas testosteroni
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Acinetobacter sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas oleovorans
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Sphingobium yanoikuyae
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas putida third step in biphenyl degradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Comamonas testosteroni
-
-
-
Cupriavidus necator
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas oleovorans
-
-
-
Pseudomonas putida Q52032
-
-
Sphingobium yanoikuyae
-
 formerly Pseudomonas paucimobilis strain Q1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli SG13009(pREP4)(pAKC1) Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloro-2,3-dihydroxybiphenyl + O2
-
 Comamonas testosteroni 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
4-chloro-2,3-dihydroxybiphenyl + O2
-
 Pseudomonas putida 3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
4-chlorocatechol + O2
-
 Pseudomonas oleovorans 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
4-chlorocatechol + O2
-
 Sphingobium yanoikuyae 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
4-chlorocatechol + O2
-
 Pseudomonas putida 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2
-
 Pseudomonas oleovorans 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2
-
 Sphingobium yanoikuyae 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
4-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas aeruginosa 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Cupriavidus necator 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Comamonas testosteroni 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Acinetobacter sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas oleovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Sphingobium yanoikuyae 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas putida 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 third step in biphenyl degradation pathway Pseudomonas putida 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
catechol + O2
-
 Pseudomonas oleovorans ?
-
 ?
catechol + O2
-
 Sphingobium yanoikuyae ?
-
 ?
catechol + O2
-
 Pseudomonas putida ?
-
 ?
additional information
-
 Sphingobium yanoikuyae ?
-
 ?
additional information strain 3,4-dihydroxybiphenyl is no substrate Comamonas testosteroni ?
-
 ?
additional information strain 3,4-dihydroxybiphenyl is no substrate Pseudomonas putida ?
-
 ?

Subunits

Subunits Comment Organism
octamer
-
 Pseudomonas oleovorans
octamer 8 * 34000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 65 loses 47% of the activity at 37°C and all activity at 65°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Pseudomonas putida