Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.39 extracted from

  • Eltis, L.D.; Hofmann, B.; Hecht, H.J.; Luensdorf, H.; Timmis, K.N.
    Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase (1993), J. Biol. Chem., 268, 2727-2732.
    View publication on PubMed
Search for more literature for 1.13.11.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas sp.
bhpC gene hyperexpressed in strain LB400 with pLEBD4 Burkholderia cepacia

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals grown from ammoinum sulfate and polyethylene glycol 6000, space groups of P42212 and I222 Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dihydroxybiphenyl
-
 Burkholderia cepacia
2,3-Dihydroxybiphenyl enzyme ist strongly inhibited by substrate, Kss 0.0003 M Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 2,3-Dihydroxybiphenyl
-
 Pseudomonas sp.
0.19
-
 3-methylcatechol
-
 Pseudomonas sp.
0.54
-
 catechol
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33200
-
 8 * 33200, SDS-PAGE Pseudomonas sp.
265000
-
 electron microscopy Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Burkholderia cepacia
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Pseudomonas sp. aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 Rhodococcus globerulus aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
 organism reclassified, prior refered as Pseudomonas sp. strain LB400 or Pseudomonas cepacia strain LB400
-
Pseudomonas sp.
-
-
-
Rhodococcus globerulus
-
 formerly Acinetobacter sp. strain P6
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
191
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
3-methylcatechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-methylcatechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
biphenyl-2,3-diol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
catechol + O2
-
 Burkholderia cepacia ?
-
 ?
catechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 33200, SDS-PAGE Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Pseudomonas sp.