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Literature summary for 1.13.11.38 extracted from
- Heterologous expression and characterization of two 1-hydroxy-2-naphthoic acid dioxygenases from Arthrobacter phenanthrenivorans (2012), Appl. Environ. Microbiol., 78, 621-627.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene diox1, gene diox1 is found on an indigenous Sphe3 plasmid, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic tree. The relative RNA transcription level of the chromosomal (diox2) gene is significantly higher than that of its plasmid (diox1) homol, overexpression in Escherichia coli | Pseudarthrobacter phenanthrenivorans |
| gene diox2, diox2 is located on the chromosome, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic tree. The relative RNA transcription level of the chromosomal (diox2) gene is significantly higher than that of its plasmid (diox1) homol, overexpression in Escherichia coli | Pseudarthrobacter phenanthrenivorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| o-phenanthroline | inhibits at 0.01 mM, addition of 0.1 mM FeSO4 restores the enzyme activity; inhibits at 0.01 mM, addition of 0.1 mM FeSO4 restores the enzyme activity | Pseudarthrobacter phenanthrenivorans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Pseudarthrobacter phenanthrenivorans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Sphe3 1-H2NA dioxygenase is iron-dependent | Pseudarthrobacter phenanthrenivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, recombinant Diox1, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
| 42000 | - |
x * 42000, recombinant Diox2, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
| 45000 | - |
x * 42000, recombinant Diox1, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
| 45000 | - |
x * 42000, recombinant Diox2, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
| 180000 | - |
gel filtration | Pseudarthrobacter phenanthrenivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-2-naphthoate + O2 | Pseudarthrobacter phenanthrenivorans | - |
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | - |
? | |
| 1-hydroxy-2-naphthoate + O2 | Pseudarthrobacter phenanthrenivorans Sphe3 | - |
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudarthrobacter phenanthrenivorans | B0BK98 | gene diox1; sp. nov., isolated from a Greek creosote-oil-polluted site, gene diox1 | - |
| Pseudarthrobacter phenanthrenivorans | B0BK99 | gene diox2; sp. nov., isolated from a Greek creosote-oil-polluted site, gene diox2 | - |
| Pseudarthrobacter phenanthrenivorans Sphe3 | B0BK98 | gene diox1; sp. nov., isolated from a Greek creosote-oil-polluted site, gene diox1 | - |
| Pseudarthrobacter phenanthrenivorans Sphe3 | B0BK99 | gene diox2; sp. nov., isolated from a Greek creosote-oil-polluted site, gene diox2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange and hydrophobic interaction chromatography, and gel filtration | Pseudarthrobacter phenanthrenivorans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain Sphe3 grows on 2-carboxybenzaldehyde, protocatechuate, and phthalate as sole carbon sources, all intermediates of phenanthrene degradation via the phthalate pathway, whereas no growth is observed on salicylic acid | Pseudarthrobacter phenanthrenivorans | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, 1 mM DTT, 48 h, stable | Pseudarthrobacter phenanthrenivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-2-naphthoate + O2 | - |
Pseudarthrobacter phenanthrenivorans | (3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | - |
? | |
| 1-hydroxy-2-naphthoate + O2 | - |
Pseudarthrobacter phenanthrenivorans Sphe3 | (3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | - |
? | |
| additional information | no activity with 3-hydroxy-2-naphthoic acid | Pseudarthrobacter phenanthrenivorans | ? | - |
? | |
| additional information | no activity with 3-hydroxy-2-naphthoic acid | Pseudarthrobacter phenanthrenivorans Sphe3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, recombinant Diox1, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
| ? | x * 42000, recombinant Diox2, x * 45000, native enzyme, SDS-PAGE | Pseudarthrobacter phenanthrenivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-H2NA dioxygenase | - |
Pseudarthrobacter phenanthrenivorans |
| 1-hydroxy-2-naphthoate dioxygenase | - |
Pseudarthrobacter phenanthrenivorans |
| 1-hydroxy-2-naphthoic acid dioxygenase | - |
Pseudarthrobacter phenanthrenivorans |
| Diox1 | - |
Pseudarthrobacter phenanthrenivorans |
| Diox2 | - |
Pseudarthrobacter phenanthrenivorans |
| Sphe3 1-H2NA dioxygenase | - |
Pseudarthrobacter phenanthrenivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudarthrobacter phenanthrenivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudarthrobacter phenanthrenivorans |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudarthrobacter phenanthrenivorans | gene diox1 is subject to carbon catabolite repression | down |
| Pseudarthrobacter phenanthrenivorans | gene diox2 is subject to carbon catabolite repression | down |
| Pseudarthrobacter phenanthrenivorans | gene diox1 is induced by the presence of phenanthrene used as a sole carbon and energy source | up |
| Pseudarthrobacter phenanthrenivorans | gene diox2 is induced by the presence of phenanthrene used as a sole carbon and energy source | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | occurrence of two diox1 and diox2 homologues in the Sphe3 genome implies that a replicative transposition event has contributed to the evolution of 1-H2NA dioxygenase in Arthrobacter phenanthrenivorans | Pseudarthrobacter phenanthrenivorans |
| metabolism | the enzyme catalyzes a step in phenanthrene degradation, overview | Pseudarthrobacter phenanthrenivorans |
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